Molecular and Cytogenetic Study of the Structure of Human Chromosome 9 in Human-CHO Somatic Cell Hybrids Using Exogenous Dominant Selectable Markers: ROW Planning Grant

使用外源显性选择标记对人类 CHO 体细胞杂交体中人类 9 号染色体结构进行分子和细胞遗传学研究：ROW Planning Grant

• 批准号: 8908619
• 负责人: HonFong Mark
• 金额: $ 1.2万
• 依托单位: Rhode Island Hospital
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8908619&HistoricalAwards=false
• 关键词: Molecular; Cytogenetic; Study; Structure; Human

This research will yield important information concerning the physical map of human chromosome 9. It will allow evaluation of the randomness of retroviral integrations into this chromosome. The cell lines produced by the research will be extremely useful in mapping the human genome. The determination of a physical map requires the ability to isolate regions of analyzable size and currently available hybrids are not adequate for this type of analysis. The incorporation of a selectable marker into the human chromosome means that the radiation hybrids containing fragments of this chromosome should be "cleaner" than those hybrids created without being able to select for the fragment of interest. One of the goals of this research is to see whether these hybrids are really superior and to evaluate the efficacy of using retroviral vectors to multiply mark a chromosome. Hybrids which have been fully characterized will yield important information on biological organization, structure and control of this portion of the genome. Comparisons with homologous regions in other species will yield important information on evolution in eukaryotic organisms. The approach adopted for chromosome 9 can be applied to any other human chromosome, or chromosomes of any other species so that the ROW Research Planning grant has considerable generality.

这项研究将产生重要的信息， 人类9号染色体的物理图谱。 它将允许评估 逆转录病毒整合到这条染色体上的随机性。 这项研究产生的细胞系将非常有用 绘制人类基因组图谱。 物理地图的确定 需要能够隔离可分析大小的区域， 目前可用的混合动力车不适合这种类型的车辆， 分析. 将选择性标记物掺入到细胞中， 人类染色体意味着含有 这条染色体的片段应该比那些 在没有选择的情况下创造的混合体 兴趣 这项研究的目的之一是看看是否 这些混合物确实是上级的， 使用逆转录病毒载体来标记染色体。 杂交种 这些已经被充分表征的技术将产生重要的 生物组织、结构和控制信息 这部分基因组。 与同源区域的比较 在其他物种的进化中将产生重要的信息， 真核生物 9号染色体采用的方法可以 适用于任何其他人类染色体，或任何 其他物种，以便ROW研究规划补助金 相当普遍。