Molecular and Cytogenetic Study of the Structure of Human Chromosome 9 in Human-CHO Somatic Cell Hybrids Using Exogenous Dominant Selectable Markers: ROW Planning Grant

这项研究将提供有关人类9号染色体物理图谱的重要信息。它将允许评估逆转录病毒整合到该染色体的随机性。这项研究产生的细胞系将在绘制人类基因组图方面非常有用。物理图谱的确定需要能够分离出可分析大小的区域，而目前可用的杂交体不足以进行这种类型的分析。在人类染色体中加入一个可选择的标记意味着，包含该染色体片段的辐射杂交应该比那些不能选择感兴趣的片段的杂交更加“干净”。这项研究的目标之一是看看这些杂交是否真的更好，并评估使用逆转录病毒载体对染色体进行倍增标记的效果。已经完全确定特征的杂交将产生关于这部分基因组的生物组织、结构和控制的重要信息。与其他物种的同源区域进行比较将产生关于真核生物进化的重要信息。对9号染色体采用的方法可以适用于任何其他人类染色体，或任何其他物种的染色体，因此ROW研究计划拨款具有相当大的普遍性。

This research will yield important information concerning the physical map of human chromosome 9. It will allow evaluation of the randomness of retroviral integrations into this chromosome. The cell lines produced by the research will be extremely useful in mapping the human genome. The determination of a physical map requires the ability to isolate regions of analyzable size and currently available hybrids are not adequate for this type of analysis. The incorporation of a selectable marker into the human chromosome means that the radiation hybrids containing fragments of this chromosome should be "cleaner" than those hybrids created without being able to select for the fragment of interest. One of the goals of this research is to see whether these hybrids are really superior and to evaluate the efficacy of using retroviral vectors to multiply mark a chromosome. Hybrids which have been fully characterized will yield important information on biological organization, structure and control of this portion of the genome. Comparisons with homologous regions in other species will yield important information on evolution in eukaryotic organisms. The approach adopted for chromosome 9 can be applied to any other human chromosome, or chromosomes of any other species so that the ROW Research Planning grant has considerable generality.