MOLECULAR CYTOGENETIC STUDY OF LUNG CANCER
肺癌的分子细胞遗传学研究
基本信息
- 批准号:2114132
- 负责人:
- 金额:$ 7.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:1996
- 资助国家:美国
- 起止时间:1996-08-05 至 1997-07-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
This study is based upon the epidemiologic, molecular and cytogenetic data from an ongoing case-control study of lung cancer in minority populations. Mutagen sensitivity, based on quantifying bleomycin-induced chromatid breaks, was associated with significantly increased lung cancer risk. We have also reported that these breaks seem to occur preferentially on chromosome 5. We now plan to extend these cytogenetic findings in peripheral lymphocytes to target (lung) tissue using metaphase preparations, cultured tumor cells, paraffin sections, and frozen tumor tissue which have already been processed and stored. The specific aims are:
1) To compare paired specimens of lung tumor and adjacent normal tissue with lymphocytes from 60 lung cancer patients to assess concordance of abnormalities on chromosome 5 using chromosome painting probes as well as with PCR-based microsatellite repeat polymorphism (MSRP) assay on Sq. The working hypothesis is that the predilection of Sq to mutagenic damage may be a marker of susceptibility to lung cancer.
2) To confirm our preliminary findings that the survival rate of cells with Sq aberrations in cases is significantly higher than that in controls, using whole chromosome 5 painting probe in 100 lung cancer cases and 100 frequency matched controls. The hypothesis being tested is that only those breakpoints that contain the loci of important genes and continue to survive and proliferate in the target tissue could contribute to neoplastic transformation.
3) To assess the associations between our findings from specific aims 1 and 2 with cigarette smoking, occupational exposure, family history and lung cancer risk by integrating epidemiologic data with the cytogenetic, molecular cytogenetic and molecular genetic (including metabolic polymorphism) data. We will test the hypothesis that cancer risk is increased in individuals who have one or more susceptible genetic trait and the relevant carcinogenic exposures. The long term goal is to further the understanding of the mechanism of mutagen sensitivity and its implication for lung carcinogenesis.
这项研究基于正在进行的少数族裔人群肺癌病例对照研究的流行病学、分子和细胞遗传学数据。诱变剂敏感性,基于对博莱霉素诱导的染色单体断裂的量化,与显著增加的肺癌风险相关。我们还报道了这些断裂似乎优先发生在5号染色体上。我们现在计划将外周淋巴细胞中的这些细胞遗传学发现扩展到靶(肺)组织,使用中期准备、培养的肿瘤细胞、石蜡切片和已经处理和储存的冰冻肿瘤组织。具体目标是:
1)将60例肺癌患者的淋巴细胞与肺癌组织及癌旁正常组织的配对标本进行比较,用染色体涂染探针检测5号染色体异常,并与Sq基因的微卫星重复序列分析(MSRP)比较。工作假说是,Sq对突变损伤的偏好可能是肺癌易感性的一个标志。
2)用5号染色体全染色探针对100例肺癌患者和100例频率匹配的正常对照进行检测,以证实我们的初步发现,即SQ异常的细胞存活率在病例组显著高于对照组。正在测试的假设是,只有那些包含重要基因位点并在目标组织中继续存活和增殖的断裂点才可能有助于肿瘤转化。
3)通过将流行病学数据与细胞遗传学、分子细胞遗传学和分子遗传学(包括代谢多态)数据相结合,评估我们在特定目标1和2中的发现与吸烟、职业暴露、家族史和肺癌风险之间的关系。我们将测试这一假设,即具有一个或多个易感基因特征和相关致癌暴露的个人患癌症的风险增加。长期目标是进一步了解诱变剂敏感性的机制及其在肺癌发生中的意义。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Xifeng Wu其他文献
Xifeng Wu的其他文献
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{{ truncateString('Xifeng Wu', 18)}}的其他基金
P-2: Risk Prediction of platinum-based chemotherapy and Radiotherapy Outcome
P-2:铂类化疗和放疗结果的风险预测
- 批准号:
8731333 - 财政年份:2013
- 资助金额:
$ 7.4万 - 项目类别:
Molecular pathways linking obesity and RCC tumorigenesis (PQ1)
连接肥胖和肾细胞癌肿瘤发生的分子途径 (PQ1)
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8383276 - 财政年份:2012
- 资助金额:
$ 7.4万 - 项目类别:
Molecular pathways linking obesity and RCC tumorigenesis (PQ1)
连接肥胖和肾细胞癌肿瘤发生的分子途径 (PQ1)
- 批准号:
8538909 - 财政年份:2012
- 资助金额:
$ 7.4万 - 项目类别:
Molecular pathways linking obesity and RCC tumorigenesis (PQ1)
连接肥胖和肾细胞癌肿瘤发生的分子途径 (PQ1)
- 批准号:
8686604 - 财政年份:2012
- 资助金额:
$ 7.4万 - 项目类别:
4th Meeting of the International Consortium of Bladder Cancer (ICBC)
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8006232 - 财政年份:2010
- 资助金额:
$ 7.4万 - 项目类别:
Lung Cancer Chemoradiation: Predictors of Survival
肺癌放化疗:生存的预测因素
- 批准号:
7939475 - 财政年份:2009
- 资助金额:
$ 7.4万 - 项目类别:
P-2: Risk Prediction of platinum-based chemotherapy and Radiotherapy Outcome
P-2:铂类化疗和放疗结果的风险预测
- 批准号:
7921399 - 财政年份:2009
- 资助金额:
$ 7.4万 - 项目类别:
Genome-Wide Association Analysis of Bladder Cancer
膀胱癌的全基因组关联分析
- 批准号:
7935041 - 财政年份:2009
- 资助金额:
$ 7.4万 - 项目类别:
Genome-Wide Association Analysis of Bladder Cancer
膀胱癌的全基因组关联分析
- 批准号:
8054297 - 财政年份:2008
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7729505 - 财政年份:2008
- 资助金额:
$ 7.4万 - 项目类别:
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