Molecular Cytogenetic Study of the Tarsier

眼镜猴（Tarsius）的进化和生殖一直存在争议。在本研究中，我们试图进行分子细胞遗传学研究，以探讨该属的系统发育位置。我们培养血细胞、淋巴细胞和肾细胞制备染色体标本。通过添加BrdU，获得了具有复制带的中期染色体。(1)确定了银汉鱼的二倍体染色体数目为80。(2)人类X染色体特异的绘画探针与整个眼镜猴X染色体杂交，表明其保守性。Y染色体长臂上有一种特异性的重复序列，这些重复序列与人类Y染色体上的DYZ 1不同源。人SRY探针与该物种Y染色体短臂的端粒部分杂交。(3)一些人类染色体特异的绘画探针揭示了人类眼镜猴同线关系。(4)我们建立了眼镜猴肾细胞系。(5)建立了眼镜猴基因组DNA文库。(6)为了比较染色体定位，我们收集了人和小鼠的cDNA克隆。首先，这些克隆被精确地定位到人类染色体上。用荧光原位杂交技术将克隆定位在眼镜猴染色体上。这些数据将有助于确定眼镜猴和人类染色体之间的同线关系。

The evolution and phylogeny of the Tarsier (Genus Tarsius) have long been controvertial. In the present study, we attempted to perform molecular cytogenetic studies to explore the phylogenetic position of this genus. We cultured blood cell lymphocytes and kidney cells to prepare chromosome preparations. By addition of BrdU, metaphase chromosomes with replication bands were obtained. (1) We confirmed that the diploid chromosome number of T.Bancanus is 80. (2) Human X chromosome-specific painting probe hybridized with the whole tarsier X chromosome, suggesting its conservatism. The Y chromosome contains a species- specific repetitive sequences on its long arm. These repetitive sequences are not homologous with human DYZ1 on the Y choromosome. Human SRY probe hybridized to the telomeric portion of the short arm of the Y chromosome of this species. (3) Some human chromosome-specific painting probes revealed human-tarsier syntenic relationships. (4) We established tarsier kidney cell lines. (5) Tarsier genomic DNA library was established. (6) For comparative chromosome mapping, we collected human and mouse cDNA clones. First, these clones were precisely mapped to human chromosomes. Then the clones were licalized on tarsier chromosomes by fluorescence in situ hybridization. The data will be useful for determining the syntenic relationship between tarsier and human chromosomes.