Development of Methodology to Determine in Vivo Metabolic Rates in Mammalian Cells as a Tool to Evaluate and Improve Bioreactor Performance

开发确定哺乳动物细胞体内代谢率的方法作为评估和提高生物反应器性能的工具

• 批准号: 8910206
• 负责人: Jacqueline Shanks
• 金额: $ 6.87万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1992-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8910206&HistoricalAwards=false
• 关键词: Development; Methodology; Determine; Vivo; Metabolic

To date, the optimization of mammalian cell bioreactor performance has been implemented via the application of empirical correlations relating easily observable cellular kinetics to medium composition and bioreactor operation. While moderately successful, there is a limit to the success that may be achieved with such an empirical approach. A greater degree of success maybe obtained by selectively altering the activity of the metabolic pathways of the cells in a manner that increases bioreactor performance. However, it is difficult to alter pathway activities in a rational fashion if the regulatory characteristics of these pathways are not known, or, more importantly, if they can not even be accurately measured. The development of a method to measure in vivo metabolic activities, as presented in this proposal, addresses this limitation. This method will provide a tool for mammalian cell technologists to approach process optimization from a different and more knowledgeable direction.

到目前为止，哺乳动物细胞生物反应器性能的优化已经完成。 通过应用经验相关性来实现， 培养基组成和生物反应器的易于观察的细胞动力学 操作 虽然适度成功，但成功是有限的。 这是可以通过这种经验方法实现的。 更大程度 成功的可能性是通过选择性地改变细胞的活性来获得的。 以增加生物反应器的方式改变细胞的代谢途径 性能 然而，很难在一个特定的环境中改变途径活性。 合理的方式，如果这些途径的监管特点是 不知道，或者，更重要的是，如果他们甚至不能准确地 测定了体内代谢产物测定方法的建立 本提案中提出的活动解决了这一局限性。 这种方法将为哺乳动物细胞技术人员提供一种工具， 从一个不同的，更有知识的方法来优化过程 方向