Probing Cellular Processes for Productivity Enhancement in Hairy Root Cultures of C. roseus

制药和精细化学品的生产（“二级 代谢物”）的植物组织培养物的方法已经被认识到， 作为一个有价值的目标多年来，但潜力 这种技术的应用是有限的。 一个主要原因是， 细胞培养的遗传不稳定性 大规模生产。 一种新型植物培养系统 是“毛状”根培养物。 私家侦探已经在她的 实验室生产和稳定的“毛”根培养， 长春花（长春花）。 拟议研究 包括研究毛状根的新陈代谢 文化，以便了解限制 次级代谢产物的产生。 生长动力学和 生物碱生产将通过高性能监测 不同毛状根的液相色谱分析 在烧瓶培养中的克隆。 生理状态和 将监测细胞培养物的碳流 在灌注反应器中通过31P和13C Nuclear 磁共振（NMR）光谱。 这种双重方法 将增强对代谢的基本理解 限制生物碱产生的细胞过程。

Production of pharmaceutical and fine chemicals ("secondary metabolites") from plant tissue culture has been recognized as a worthwhile objective for many years, but the potential of this technology has been limited. A major reason is the genetic instability of cell culture for continuous production on a large scale. A novel plant culture system is "hairy" root cultures. The PI has established in her laboratory productive and stable "hairy" root cultures for Catharanthus roseus (periwinkle). The proposed research involves investigating the metabolism of hairy root cultures in order to understand the factors which limit production of secondary metabolites. Growth kinetics and alkaloid production will be monitored by high performance liquid chromatography (HPLC) for different hairy root clones in flask cultures. The physiological state and carbon flows of the cell culture will be monitored noninvasively in a perfusion reactor by 31P and 13C Nuclear Magnetic Resonance (NMR) spectroscopy. This dual approach will enhance fundamental understanding of the metabolic cellular processes limiting alkaloid production.