Peroxisome Biogenesis in the Yeasts Candida tropicalis and Saccharomyces cerevisiae

热带假丝酵母和酿酒酵母中过氧化物酶体的生物发生

• 批准号: 8916459
• 负责人: Gillian Small
• 金额: --
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-15 至 1991-08-20
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8916459&HistoricalAwards=false
• 关键词: Peroxisome; Biogenesis; Yeasts; Candida; tropicalis

Peroxisomes are nearly ubiquitous in eukaryotic cells and have essential functions in respiration and lipid metabolism. Peroxisomal proteins are nuclear-encoded and are synthesized on free polyribosomes, then imported into pre-existing peroxisomes post-translationally. Peroxisomal proteins, unlike many mitochondrial and chloroplast proteins, are synthesized at their mature size and lack cleavable transit peptides. The long term objective of this project is to understand the mechanism of peroxisome assembly. The aims of this proposal are to identify topogenic sequences that act to direct peroxisomal proteins to yeast peroxisomes. Previously, this laboratory determined conditions for measuring the in vitro import of peroxisomal proteins to peroxisomes from the yeast Candida tropicalis, and identified topogenic sequences in one peroxosomal protein (aceyl-CoA oxidase) that are capable of direction this protein to peroxisomes. Nothing is known regarding the targeting and insertion of peroxisomal integral membrane proteins. The plan is to clone and sequence a matrix and membrane protein from C. tropicalis peroxisomes. Using the established in vitro assay and by an in vivo import assay, peroxisomal targetting signals will be investigated and compared. Also, the gene encloding peroxisomal citrate synthase from Saccharomyces cerevisiae is in hand; this will be expressed and, using the above methods, it will be determined whether signal sequences are conserved in peroxisomal proteins from C. tropicalis and S. cerevisiae. By these means, it is hoped to gain a better understanding of the mechanism by which peroxisomes are assembled.

过氧化物酶体几乎普遍存在于真核细胞中， 在呼吸和脂质代谢中起重要作用。 过氧化物酶体蛋白是核编码的， 游离的多聚核糖体，然后输入到预先存在的过氧化物酶体中 事后的 过氧化物酶体蛋白，不像许多 线粒体和叶绿体蛋白质，是在它们的 成熟的大小和缺乏可裂解的转运肽。 长期 本项目的目的是了解 过氧化物酶体组装 本提案的目的是确定 局部基因序列，其作用是指导过氧化物酶体蛋白， 酵母过氧化物酶体 此前，该实验室确定 过氧化物酶体体外输入测定条件 蛋白质转化为来自酵母热带假丝酵母的过氧化物酶体，和 在一种过氧化物酶体蛋白中鉴定出的拓扑序列 （乙酰辅酶A氧化酶），能够指导这种蛋白质， 过氧化物酶体 目前还不清楚目标是什么， 过氧化物酶体整合膜蛋白的插入。 该计划是 克隆并测序C. 热带木过氧化物酶体 使用已建立的体外试验和 通过体内输入测定，过氧化物酶体靶向信号将 进行调查和比较。 此外，基因表达 来自酿酒酵母的过氧化物酶体柠檬酸合酶是 手;这将被表达，并使用上述方法，它 将确定信号序列是否保守， 过氧化物酶体蛋白C. tropicalis和S.啤酒。 通过这些手段，希望能更好地了解 过氧化物酶体组装的机制。