Fluorescence Studies of Dynamic Processes in Peroxidase hemeprotein

过氧化物酶血红素蛋白动态过程的荧光研究

• 批准号: 8916623
• 负责人: David Jameson
• 金额: $ 1.7万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-15 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8916623&HistoricalAwards=false
• 关键词: Fluorescence; Studies; Dynamic; Processes; Peroxidase

This award supports cooperative research in biophysics to be conducted by David Jameson of the University of Hawaii and Juan Brunet of the Catholic University of Valparaiso, Chile. This work will continue research in fluorescent spectroscopic studies of the hemeprotein, horseradish peroxidase. It will elucidate the dynamics of the protein matrix near the heme binding site, particularly relaxation processes. An effort will be made to show the functional significance of protein molecular motions to enzyme catalysis and the energetics of ligand binding. Peroxidases catalyze the oxidation of various organic and inorganic compounds and are thus an attractive model for protein dynamic studies. This work continues a successful and productive collaboration between Dr. Jameson who is expert in fluorescence spectroscopy, instrumentation and theory of fluorescence and Dr. Brunet, a well-trained physical chemist who has funding for a new fluorometer, partly as a result of the collaboration. This and the training that graduate students are receiving in Chile illustrate the advantages of the Science in Developing Countries program.

该奖项支持夏威夷大学的David Jameson和智利瓦尔帕莱索天主教大学的Juan Brunet在生物物理学方面进行的合作研究。这项工作将继续研究血红蛋白，辣根过氧化物酶的荧光光谱研究。它将阐明血红素结合部位附近蛋白质基质的动力学，特别是松弛过程。将努力展示蛋白质分子运动对酶催化的功能意义和配基结合的能量学。过氧化物酶催化各种有机和无机化合物的氧化，因此是蛋白质动力学研究的一个有吸引力的模型。这项工作延续了詹姆逊博士和布鲁内特博士之间成功而富有成效的合作。詹姆森博士是荧光光谱学、仪器和荧光理论方面的专家，布鲁内特博士是一名训练有素的物理化学家，他资助了一台新的荧光仪，这在一定程度上是合作的结果。这一点和研究生在智利接受的培训说明了发展中国家的科学计划的优势。