Conformational Dynamics of Components of Protein Biosynthesis Systems

本研究的目的是研究来自细菌和高等生物的蛋白质生物合成系统组分的构象动力学。具体来说，将以分离形式和与各自蛋白质生物合成系统中适当成分的复合物研究来自大肠杆菌和小牛脑的延伸因子。在大肠杆菌蛋白的情况下，复合物将是鸟嘌呤核苷酸结合形式，与另一种延伸因子形成的异二聚体，与鸟嘌呤核苷酸和氨基酰化trna形成的三元复合物以及与抗生素氧化还原形成的复合物。小牛脑的延伸因子也将与鸟嘌呤核苷酸和其他延伸因子的配合物进行研究。这些研究的目标将是检查这些不同的生物重要复合物形成时发生的构象改变的性质和程度，以及阐明这些复合物的流体动力学方面。除了提供这些蛋白质生物合成系统的重要组成部分的信息外，拟议的研究还将与其他奎宁核苷酸结合蛋白的相关研究相关。

The object of this study is the investigation of the conformational dynamics of components of protein biosynthesis systems from bacterial and higher organisms. Specifically, elongation factors from c. coli and from calf brain will be studied both in isolated forms and in complexes with appropriate components from their respective protein biosynthesis systems. In the case of the E. coli protein, the complexes will be the guanine nucleotide bound forms, the heterodimer formed with still another elongation factor, the ternary complex formed with guanine nucleotide and aminoacylated-tRNA and the complex formed with the antibiotic aurodox. The elongation factor from calf brain will also be studied in its complexes with guanine nucleotides and with other elongation factors. The goal of these studies will be to examine the nature and extent of the conformational alterations which occur upon formation of these various biologically important complexes as well as to elucidate hydrodynamic aspects of the complexes. In addition to providing information on these important components of protein biosynthesis systems the proposed studies will also have relevance to related research on other quinine nucleotide binding proteins.