Conformational Dynamics of Components of Protein Biosynthesis Systems

蛋白质生物合成系统成分的构象动力学

• 批准号: 9005195
• 负责人: David Jameson
• 金额: $ 32.1万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-08-01 至 1994-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9005195&HistoricalAwards=false
• 关键词: Conformational; Dynamics; Components; Protein; Biosynthesis

The object of this study is the investigation of the conformational dynamics of components of protein biosynthesis systems from bacterial and higher organisms. Specifically, elongation factors from c. coli and from calf brain will be studied both in isolated forms and in complexes with appropriate components from their respective protein biosynthesis systems. In the case of the E. coli protein, the complexes will be the guanine nucleotide bound forms, the heterodimer formed with still another elongation factor, the ternary complex formed with guanine nucleotide and aminoacylated-tRNA and the complex formed with the antibiotic aurodox. The elongation factor from calf brain will also be studied in its complexes with guanine nucleotides and with other elongation factors. The goal of these studies will be to examine the nature and extent of the conformational alterations which occur upon formation of these various biologically important complexes as well as to elucidate hydrodynamic aspects of the complexes. In addition to providing information on these important components of protein biosynthesis systems the proposed studies will also have relevance to related research on other quinine nucleotide binding proteins.

本研究的目的是研究细菌和高等生物体蛋白质生物合成系统成分的构象动力学。 具体来说，伸长因子来自c。大肠杆菌和小牛脑将以分离形式和与各自蛋白质生物合成系统中适当成分的复合物形式进行研究。 就大肠杆菌蛋白而言，复合物将是鸟嘌呤核苷酸结合形式、与另一种延伸因子形成的异二聚体、与鸟嘌呤核苷酸和氨酰化-tRNA形成的三元复合物以及与抗生素Aurodox形成的复合物。 小牛脑的伸长因子也将在其与鸟嘌呤核苷酸和其他伸长因子的复合物中进行研究。 这些研究的目的是检查这些各种具有重要生物学意义的复合物形成时发生的构象改变的性质和程度，并阐明这些复合物的流体动力学方面。 除了提供蛋白质生物合成系统这些重要组成部分的信息之外，拟议的研究还将与其他奎宁核苷酸结合蛋白的相关研究相关。