U.S.-Chile Cooperative Science Program: Conformational Dynamics of Horseradish Peroxidase

该美洲计划奖将支持夏威夷大学的David Jameson博士和智利瓦尔帕莱索天主教大学的Juan Brunet博士在生物化学方面的合作研究。这项研究的目的是利用稳态和时间分辨荧光方法来表征和量化辣根过氧化物酶(HRP)的动态方面，并研究它们与各种催化过程的关系。这些光谱研究对于与在其他血红素酶中获得的类似信息进行比较将是重要的。辣根过氧化物酶是一种血红蛋白，能催化多种有机和无机化合物的氧化和过氧化，是蛋白质动力学研究的一个有吸引力的模型。这项工作延续了两位科学家之间成功而富有成效的合作。以前的研究中没有提出时间分辨荧光测量，因此，本研究的结果将为HRP的结构和功能提供有用的新信息。

This Americas Program award will support collaborative research in biochemistry between Dr. David Jameson of the University of Hawaii and Dr. Juan Brunet of the Catholic University of Valparaiso, Chile. The objective of the research is to characterize and quantify dynamic aspects of Horseradish Peroxidase (HRP), using steady-state and time-resolved fluorescence methodologies, and investigate their relationship to various catalytic processes. These spectroscopic investigations will be important for comparison with similar information obtained in other heme enzymes. Horseradish Peroxidase is a hemeprotein, which catalyses the oxidation and peroxidation of a variety of organic and inorganic compounds and is an attractive model for protein dynamic studies. This work continues a successful and productive collaboration between the two scientists. The proposed time-resolved fluorescence measurements have not been included in previous studies, thus, the results of this research will provide useful new information about the structure and function of the HRP.