Crystal Structures of BMH: CaM-M13 and 83-84Calmodulin

BMH 的晶体结构：CaM-M13 和 83-84 钙调蛋白

• 批准号: 8917285
• 负责人: Robert Kretsinger
• 金额: $ 40.5万
• 依托单位: University of Virginia Main Campus
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-15 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8917285&HistoricalAwards=false
• 关键词: Crystal; Structures; BMH; CaM; M13

The protein calmodulin is the principle transducer of messenger calcium. It is believed to exist within the cytosols of all cells of all eukaryotes. In its calcium forms, (but not in other forms) it activates at least ten and probably as many as twenty different enzymes or structural proteins. It has evolved by gene duplication and is very highly conserved especially among the animals. Previous crystal structure studies have revealed that calmodulin consists of two protein lobes joined by a short pepitde linking chain. This proposal is to study two crystal structures of proteins related to calmodulin. Crystals suitable for x-ray diffraction studies have already been grown. The significance of these determinations is their test of the model in which the central linker of calmodulin functions as a flexible tether allowing specific patches on the two labels to enfold a target.

蛋白质钙调素是信使的主要转换器 钙 它被认为存在于所有的细胞质中， 所有真核生物的细胞。 以其钙的形式，（但不是在其他 形式），它激活至少十个，可能多达二十个 不同的酶或结构蛋白。 它是由基因进化而来的 重复，并且非常高度保守，特别是在 动物 先前的晶体结构研究表明， 钙调素由两个蛋白质叶组成， 肽连接链。 本建议是研究两种晶体 与钙调素相关的蛋白质结构。 晶体适用 用于X射线衍射的研究已经发展起来。 的 这些决定的意义在于他们对模型的检验 其中钙调蛋白的中心连接体起着柔性的 系链允许两个标签上的特定补丁包绕一个 目标