Metabolism of Isoleucine to Insect Juvenile Hormone II via Propionyl-Coenzyme A

异亮氨酸通过丙酰辅酶 A 代谢为昆虫保幼激素 II

• 批准号: 8919257
• 负责人: David Schooley
• 金额: $ 26万
• 依托单位: Board of Regents, NSHE, obo University of Nevada, Reno
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-01-01 至 1993-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8919257&HistoricalAwards=false
• 关键词: Metabolism; Isoleucine; Insect; Juvenile; Hormone

It has been shown that isoleucine and valine supply propionate units used specifically for the biosynthesis of the ethyl-branched juvenile hormones (JHs). Insect corpora allata (CA) which do not metabolize them readily (the CA of Lepidoptera) secrete propionate and ethyl-branched JHs. This research will extend these studies and determine what allows the CA to violate the rules of sesquiterpenoid biosynthesis and to incorporate propionyl-CoA into this pathway. The metabolism of ıU-14c! isoleucine will be examined in several tissues of insects which make the ethyl-branched JHs vs. those that produce only JH III. These data are important for interpreting metabolic differences between species. In addition, the metabolism of ıU-14c! isoleucine in isolated mitochondria will also be studied. This work will determine whether or not there is an efficient transport system for exporting propionyl-CoA from the mitochondria. Carnitine propionyl transferase and methyl citrate formation will be examined as likely possibilities for propionyl CoA transport. If this phenomenon cannot be explained by a unique system for propionate transport, the possibility will be investigated that certain enzymes might be located in different subcellular organelles than those in which they are generally held to exist. Juvenile hormones are a group of structurally related compounds secreted by members of the class Insecta and which possess morphogenetic and gonadotropic activity. It is important to have a basic knowledge of the metabolic pathways involved to understand why and how insects are able to synthesize these hormones. Knowledge of the biochemical details of this pathway could point the way towards synthesis of chemicals which specifically inhibit it. These chemicals would be antihormones (and probably, therefore, specific insecticides) to the order of insects that includes moths, which are some of the most damaging of crop pests.

已经表明，异亮氨酸和缬氨酸供应 丙酸单位专门用于生物合成 乙基分支保幼激素（JHS）。 昆虫体 （一）不以物易物，不以物易物; 鳞翅目）分泌丙酸和乙基分支的JH。 这 研究将扩大这些研究，并确定什么允许 CA违反倍半萜类化合物生物合成的规则 并将丙酰辅酶A掺入该途径中。 的 14 C的代谢！异亮氨酸将在几个 产生乙基支化JH的昆虫组织与 只生产JH III的那些。 这些数据对于 解释物种间的代谢差异 在 此外，代谢的<$U-14 c！分离的异亮氨酸 还将研究线粒体。 这项工作将决定 是否有一个有效的运输系统， 从线粒体输出丙酰辅酶A。 肉碱 丙酰基转移酶和柠檬酸甲酯的形成将是 作为丙酰辅酶A运输的可能性进行检查。 如果这种现象不能用一个独特的系统来解释， 丙酸运输，将调查的可能性 某些酶可能位于不同的 亚细胞器比那些他们通常是 坚持存在。 保幼激素是一组结构相关的 昆虫纲成员分泌的化合物， 具有形态发生和促性腺活性。 是 了解代谢途径的基本知识很重要 了解昆虫为什么以及如何能够 合成这些激素。 生物化学知识 这条途径的细节可以为合成 这些化学物质可以抑制它。 可能是抗激素（因此可能是特异性的， 杀虫剂）至包括蛾的昆虫目， 它们是最具破坏性的农作物害虫。