Enzyme Hydrolysis Kinetics of Pretreated Lignocellulosic Substrtrates

目前关于预处理条件对酶在木质纤维底物上的吸附和水解动力学的影响的研究将扩展到包括更多的底物和更大范围的酶浓度。纤维素酶在木质素上的吸附和稀释将受到特别关注，因为目前的工作表明，在高温下进行预处理可以降低木质素的吸附能力。建议研究前处理和随后的酶解过程中底物粒度分布的变化。计划将这些结果与孔体积测量相结合，以估计可用于酶吸附的总表面积，这是动力学模型中的一个重要参数。将开发一个协同作用的定量模型，以努力解释当前工作中的意外发现，即水解率与吸附在纤维素上的酶的0.67次方成正比，而不是线性函数。

Current studies of the effect of pretreatment conditions on the kinetics of enzyme adsorption on, and hydrolysis of, lignocellulosic substrates will be extended to include additional substrates and a wider range of enzyme concentrations. The adsorption of cellulase enzymes on, and dilution from, lignin will receive particular attention since it was shown in current work that the adsorption capacity of the lignin can be reduced by pretreatment at high temperature. It is proposed to study the changes in substrate particle size distribution that results from pretreatment and during the subsequent enzymatic hydrolysis. It is planned to combine these results with pore volume measurements in order to estimate the total surface area that is available for enzyme adsorption, an important parameter in the kinetics model. A quantitative model of synergism will be developed in an effort to explain the unexpected discovery in current work that the hydrolysis rate is proportional to the 0.67 power of the enzyme adsorbed on the cellulose rather than a linear function.