Enzyme Hydrolysis Kinetics of Pretreated Lignocellulosic Substrtrates

预处理木质纤维素底物的酶水解动力学

• 批准号: 8919335
• 负责人: Alvin Converse
• 金额: $ 18.19万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-15 至 1994-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8919335&HistoricalAwards=false
• 关键词: Enzyme; Hydrolysis; Kinetics; Pretreated; Lignocellulosic

Current studies of the effect of pretreatment conditions on the kinetics of enzyme adsorption on, and hydrolysis of, lignocellulosic substrates will be extended to include additional substrates and a wider range of enzyme concentrations. The adsorption of cellulase enzymes on, and dilution from, lignin will receive particular attention since it was shown in current work that the adsorption capacity of the lignin can be reduced by pretreatment at high temperature. It is proposed to study the changes in substrate particle size distribution that results from pretreatment and during the subsequent enzymatic hydrolysis. It is planned to combine these results with pore volume measurements in order to estimate the total surface area that is available for enzyme adsorption, an important parameter in the kinetics model. A quantitative model of synergism will be developed in an effort to explain the unexpected discovery in current work that the hydrolysis rate is proportional to the 0.67 power of the enzyme adsorbed on the cellulose rather than a linear function.

预处理条件影响的研究现状 酶吸附和水解的动力学 的木质纤维素底物将扩展到包括 额外的底物和更广泛的酶 浓度的 纤维素酶的吸附， 和稀释，木质素将得到特别的 注意，因为目前的工作表明， 木质素的吸附能力可以通过以下方式降低： 高温预处理。 提出了研究基体颗粒的变化 由预处理产生的粒度分布， 在随后的酶水解过程中。 计划 为了联合收割机将这些结果与孔体积测量结合， 以估计可用的总表面积 对于酶吸附， 动力学模型 协同作用的定量模型将在一个 努力解释目前工作中的意外发现 水解速率与0.67次方成正比 吸附在纤维素上的酶，而不是 线性函数