On the Importance of Substrate Reactivity and Enzyme Tethering in the Enzymatic Hydrolysis of Cellulosic Substrates

9523627匡威该项目的总体目标是使用新的实验技术来研究随着水解的进行纤维素水解速率的下降。 两个新的假设进行测试，以部分解释这种下降。 一种被称为链端假说，其中通过预处理产生过量的纤维素链端导致纤维二糖水解酶（CBH）对这些末端的水解比内切酶形成新末端更快。 另一种假说被称为束缚假说，一旦被吸附，CBH具有有限数量的纤维素末端，并且变得无活性，直到它在新的位点解吸和再吸附。 ***

9523627 Converse The overall objective of this project is to use new experimental techniques to study the fall-off in the hydrolysis rate of cellulose as the hydrolysis proceeds. Two new hypotheses are to be tested to partially explain this fall-off. One is referred to as the chain-end hypothesis, where production of excess cellulose chain ends by pretreatment leads to a more rapid hydrolysis of these ends by cellobiohydrolase (CBH) than formation of new ends by endogluconase. The other hypothesis is called the tethering hypothesis, where once adsorbed, CBH has a limited number of cellulose ends available to it, and becomes inactive until it desorbs and readsorbs at a fresh site. ***