On the Importance of Substrate Reactivity and Enzyme Tethering in the Enzymatic Hydrolysis of Cellulosic Substrates

底物反应性和酶束缚在纤维素底物酶水解中的重要性

• 批准号: 9523627
• 负责人: Alvin Converse
• 金额: $ 4.98万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-07-01 至 1996-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9523627&HistoricalAwards=false
• 关键词: Importance; Substrate; Reactivity; Enzyme; Tethering

9523627 Converse The overall objective of this project is to use new experimental techniques to study the fall-off in the hydrolysis rate of cellulose as the hydrolysis proceeds. Two new hypotheses are to be tested to partially explain this fall-off. One is referred to as the chain-end hypothesis, where production of excess cellulose chain ends by pretreatment leads to a more rapid hydrolysis of these ends by cellobiohydrolase (CBH) than formation of new ends by endogluconase. The other hypothesis is called the tethering hypothesis, where once adsorbed, CBH has a limited number of cellulose ends available to it, and becomes inactive until it desorbs and readsorbs at a fresh site. ***

9523627相反，这个项目的总体目标是使用新的实验技术来研究纤维素在水解过程中的水解率的下降。两个新的假设将被检验，以部分解释这种下降。一种被称为链末端假说，即通过预处理产生多余的纤维素链末端会导致纤维二糖水解酶(CBH)对这些末端的水解速度快于内切葡萄糖酶形成新末端的速度。另一种假说被称为系留假说，一旦被吸附，CBH可用的纤维素端数量有限，直到它在新的位置解吸和再吸附时才变得不活跃。***