On the Effect of Thermo-Chemical Pretreatment on the Enzymatic Hydrolysis of Insoluble Lignocellulosic Substrates

热化学预处理对不溶性木质纤维素底物酶解效果的研究

• 批准号: 8701010
• 负责人: Alvin Converse
• 金额: $ 9.4万
• 依托单位: Dartmouth College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1989-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8701010&HistoricalAwards=false
• 关键词: Effect; Thermo; Chemical; Pretreatment; Enzymatic

Narrative: This proposal targets the important problem of converting lignocellulosic substrates to liquid fuel, namely ethanol. In addition, the sugar solution resulting from enzyme hydrolysis can be used as a general fermentation substrate. An attempt will be made to quantify the kinetics of the enzymatic hydrolysis of thermo-chemically pretreated lignocellulosic material. In particular, the work will relate the physical and chemical effects of the "steam-explosion" pretreatment to the kinetics of the subsequent hydrolysis of woody biomass. Area and pore volume available to enzyme action, degree of polymerization, crystallinity index, and lignin content and morphological state will be measured in order to characterize the substrate. These variables, as well as the enzyme concentration and activity and product concentration, are to be followed throughout the hydrolysis reaction. Fundamental studies of this kind will help to lay a basis for the development of new processes for enzymatically converting insoluble lignocellulosic substrates to more valuable products, and to provide a better understanding of heterogeneous enzyme kinetics in general. The resulting mathematical models will be sensitive to process behavior and therefore be valuable in the design and development of efficient, economical production of useful products via enzymatic hydrolysis of insoluble lignocellulosic substrates.

叙述：这项提议针对的是将木质纤维底物转化为液体燃料的重要问题，即乙醇。此外，酶解产生的糖液可用作一般发酵底物。我们将尝试对经过热化学处理的木质纤维材料的酶促水解动力学进行量化。特别是，这项工作将把“蒸汽爆炸”预处理的物理和化学效应与随后木质生物质的水解动力学联系起来。将测量可用于酶作用的面积和孔体积、聚合度、结晶度指数以及木质素含量和形态状态，以表征底物。这些变量，以及酶浓度、活性和产物浓度，在整个水解反应过程中都要遵循。这类基础研究将有助于为酶转化不溶性木质纤维底物生产更有价值的产品的新工艺的开发奠定基础，并有助于从总体上更好地了解多相酶的动力学。由此得到的数学模型将对过程行为敏感，因此对于设计和开发通过酶解不溶的木质纤维底物有效、经济地生产有用产品具有重要价值。