Detailed Examination of Fate, Morphogenesis and Regulation in the Anterior Neural Plate and Ridge of Xenopus laevis

非洲爪蟾前神经板和脊的命运、形态发生和调节的详细检查

• 批准号: 8919785
• 负责人: William Harris
• 金额: $ 4.07万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8919785&HistoricalAwards=false
• 关键词: Detailed; Examination; Fate; Morphogenesis; Regulation

This researcch project will use fluorescent tagging techniques to follow the fates of cells in the anterior neural plate and ridge of Xenopus laevis. In a previous study, the investigators found that the dyes Hoechst and DiI could successfully be used as autonomous markers for small groups of cells in the neural plate. With these dyes they were able to construct a detailed fate map of the neural plate. Three aspects of this fate map will receive detailed attention in the planned research: 1) The cellular movements associated with the most anterior plate and ridge will be charted; 2) The ancestry of the anterior pituitary will be clarified; and 3) The regulatory capacities of the anterior neural plate and ridge will receive detailed examination.

本研究将利用荧光标记技术来追踪非洲爪哇前神经板和脊内细胞的命运。在之前的一项研究中，研究人员发现，染料Hoechst和DII可以成功地用作神经板中小群细胞的自主标记。使用这些染料，他们能够构建神经板的详细命运图。这张命运图的三个方面将在计划中的研究中得到详细的关注：1)与最前面的板和脊相关的细胞运动将被绘制出来；2)垂体前叶的祖先将被澄清；以及3)前神经板和脊的调节能力将得到详细的检查。