Next generation live super-resolution microscopy: development and application at the Cambridge Advanced Imaging Centre

剑桥先进成像中心（CAIC）将与国际领先的实验室合作，开发两种仪器，在分辨率、多功能性和记录深度方面超过目前可用的商业解决方案。这些仪器将使我们能够在纳米尺度上观察活组织中单个分子的运输，并确定活细胞中蛋白质复合物的结构。沃尔夫森基金会的投资以及大学基金的重大贡献使我们能够建立一个现代化的专用设施，促进物理科学家和生物学家的合作。专门的成像专家和计算机程序员将协助科学家使用新仪器和数据分析。从一开始，仪器的开发就受到最终用户需求的驱动，这些需求来自癌症、发育生物学、神经科学和神经退行性疾病的研究课题。由于CAIC拥有广泛的生物和光学物理专业知识，生物学家将能够快速测试成像平台是否适合其特定的实验要求，并确定成功执行这些实验所需的条件。该提案解决了剑桥大学世界级生物学家确定的现代生物成像中一些最迫切的需求。剑桥的生物医学科学家对癌症和神经变性等疾病的分子和细胞生物学感兴趣，生物科学家对发育生物学和神经生物学的基本问题感兴趣，CAIC将帮助物理科学家设计和改进这些显微镜，以便他们能够提出有关细胞结构和功能的最基本问题，并获得对健康和疾病中细胞功能的新见解。剑桥大学的优先倡议。新的CAIC设施将包括两个区域：多用户成像设施和一个独立的光学实验室，以允许开发需要开放激光路径安排的原型设备。CAIC与剑桥的其他成像中心之间存在着密切的联系，这使得互补的专业知识能够协同增效。CAIC不仅是剑桥大学先进显微镜的物理中心，它还是成像技术进步的基础。在光学开发领域，新的显微镜被建造和试用。从那里，他们将被转移到服务区，在那里他们使生物学家能够使用最先进的成像方法，而不需要寻找资金购买或建造这些仪器自己。但随着这种情况的发生，新的空间成为可用于建立和测试新的机器在光学开发领域。培训计划是一个关键组成部分，在长期的成功，该中心。成像技术的新发展将有助于加强生物显微镜的理论和实践方面以及图像处理技术方面的培训。研究生和博士后研究员将与物理科学家一起工作，建造新机器，生物科学家测试它们。希望这种新一代的跨学科显微镜学家能够受到启发，建立下一代先进的成像仪器。

The Cambridge Advanced Imaging Centre (CAIC) will collaborate with internationally leading laboratories to develop two instruments that surpass currently available commercial solutions in terms of resolution, versatility and depth of recording. These instruments will enable us to observe at nanometer scale the trafficking of individual molecules in living tissue and determine the structure of protein complexes in live cells. A investment from the Wolfson Foundation together with significant contributions from University funds have enabled us to build a modern, purpose-built facility that fosters collaboration of physical scientists and biologists. Dedicated staff imaging specialists and a computer programmer will assist scientists with the use of new instruments and data analysis. From the start, the instrument development is driven from end user need, arising from research topics in cancer, developmental biology, neuroscience and neurodegeneration. Due to the wide range of biological and optical physics expertise present at CAIC, biologists will be able to quickly test the suitability of imaging platforms for their specific experimental requirements and establish what will be needed to perform these experiments successfully.This proposal addresses some of the most pressing needs in modern biological imaging identified by world-class biologists at Cambridge University. Biomedical scientists at Cambridge interested in the molecular and cell biology of diseases such as cancer and neurodegeneration, and biological scientists interested in basic questions of developmental biology and neurobiology, will help physical scientists devise and refine these microscopes so that they can ask the most fundamental questions about cellular structure and function and gain new insights into cellular functions in health and disease.CAIC is a top priority initiative of the University of Cambridge. The new CAIC facility will consists of two areas: the multi-user imaging facility and a separated optics lab to allow the development of prototype equipment requiring open laser path arrangements. Strong links exist between CAIC and the other imaging hubs at Cambridge, which allows synergising on complementary expertise. CAIC is not just a physical hub for advanced microscopy at the University of Cambridge, it is the basis of a pipeline for the advancement of imaging technology. In the optical development area, new microscopes are built and trialled. From there they will be moved to the service area, where they enable biologists to use state-of-the-art imaging methods without the need to find funds to buy or build these instruments themselves. But as this happens new space becomes available to build and test new machines in the optical development area.A training programme is a key component in the long-term success of the centre. New developments in imaging technologies will feed through to enhancing training in the theoretical and practical aspects of biological microscopy, and in image processing techniques. Graduate students and postdoctoral fellows will work alongside the physical scientists building the new machines and the biological scientists trialing them. This new breed of interdisciplinary microscopists will, it is hoped, be inspired to build the next generation of advanced imaging instruments.