Next generation live super-resolution microscopy: development and application at the Cambridge Advanced Imaging Centre

下一代实时超分辨率显微镜：剑桥高级成像中心的开发和应用

• 批准号: MR/K015850/1
• 负责人: William Harris
• 金额: $ 202.68万
• 依托单位: University of Cambridge
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2013
• 资助国家: 英国
• 起止时间: 2013 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=MR%2FK015850%2F1
• 关键词: Next; generation; live; super; resolution

The Cambridge Advanced Imaging Centre (CAIC) will collaborate with internationally leading laboratories to develop two instruments that surpass currently available commercial solutions in terms of resolution, versatility and depth of recording. These instruments will enable us to observe at nanometer scale the trafficking of individual molecules in living tissue and determine the structure of protein complexes in live cells. A investment from the Wolfson Foundation together with significant contributions from University funds have enabled us to build a modern, purpose-built facility that fosters collaboration of physical scientists and biologists. Dedicated staff imaging specialists and a computer programmer will assist scientists with the use of new instruments and data analysis. From the start, the instrument development is driven from end user need, arising from research topics in cancer, developmental biology, neuroscience and neurodegeneration. Due to the wide range of biological and optical physics expertise present at CAIC, biologists will be able to quickly test the suitability of imaging platforms for their specific experimental requirements and establish what will be needed to perform these experiments successfully.This proposal addresses some of the most pressing needs in modern biological imaging identified by world-class biologists at Cambridge University. Biomedical scientists at Cambridge interested in the molecular and cell biology of diseases such as cancer and neurodegeneration, and biological scientists interested in basic questions of developmental biology and neurobiology, will help physical scientists devise and refine these microscopes so that they can ask the most fundamental questions about cellular structure and function and gain new insights into cellular functions in health and disease.CAIC is a top priority initiative of the University of Cambridge. The new CAIC facility will consists of two areas: the multi-user imaging facility and a separated optics lab to allow the development of prototype equipment requiring open laser path arrangements. Strong links exist between CAIC and the other imaging hubs at Cambridge, which allows synergising on complementary expertise. CAIC is not just a physical hub for advanced microscopy at the University of Cambridge, it is the basis of a pipeline for the advancement of imaging technology. In the optical development area, new microscopes are built and trialled. From there they will be moved to the service area, where they enable biologists to use state-of-the-art imaging methods without the need to find funds to buy or build these instruments themselves. But as this happens new space becomes available to build and test new machines in the optical development area.A training programme is a key component in the long-term success of the centre. New developments in imaging technologies will feed through to enhancing training in the theoretical and practical aspects of biological microscopy, and in image processing techniques. Graduate students and postdoctoral fellows will work alongside the physical scientists building the new machines and the biological scientists trialing them. This new breed of interdisciplinary microscopists will, it is hoped, be inspired to build the next generation of advanced imaging instruments.

剑桥高级成像中心(CAIC)将与国际领先的实验室合作开发两种仪器，在分辨率、多功能性和记录深度方面超过目前可用的商业解决方案。这些仪器将使我们能够在纳米尺度上观察单个分子在活组织中的运输，并确定活细胞中蛋白质复合体的结构。沃尔夫森基金会的投资和大学基金的重大贡献使我们能够建造一个现代化的、专门建造的设施，促进物理学家和生物学家的合作。专门的工作人员、成像专家和一名计算机程序员将协助科学家使用新仪器和数据分析。从一开始，仪器的开发就是由终端用户的需求驱动的，产生于癌症、发育生物学、神经科学和神经退行性变的研究课题。由于CAIC拥有广泛的生物和光学物理专业知识，生物学家将能够快速测试成像平台是否适合他们的特定实验要求，并确定成功执行这些实验所需的条件。这项建议解决了剑桥大学世界级生物学家确定的现代生物成像中的一些最紧迫的需求。剑桥的生物医学科学家对癌症和神经退行性疾病等疾病的分子和细胞生物学感兴趣，生物科学家对发育生物学和神经生物学的基本问题感兴趣，他们将帮助物理学家设计和改进这些显微镜，以便他们能够提出关于细胞结构和功能的最基本问题，并对细胞在健康和疾病中的功能有新的见解。CAIC是剑桥大学的首要任务。新的CAIC设施将包括两个区域：多用户成像设施和一个独立的光学实验室，以允许开发需要开放激光路径安排的原型设备。CAIC与剑桥的其他成像中心之间存在着牢固的联系，这使得它们能够在互补的专业知识上进行协同。CAIC不仅是剑桥大学先进显微镜的物理中心，也是促进成像技术进步的管道的基础。在光学发展领域，建造并试验了新的显微镜。从那里，它们将被转移到服务区，在那里，生物学家可以使用最先进的成像方法，而不需要找到资金来购买或自己建造这些仪器。但随着这种情况的发生，新的空间可以用来建造和测试光学开发领域的新机器。培训计划是该中心长期成功的关键组成部分。成像技术的新发展将有助于加强生物显微镜的理论和实践方面以及图像处理技术方面的培训。研究生和博士后研究员将与制造新机器的物理学家和试验它们的生物科学家一起工作。人们希望，这种新一代的跨学科显微镜工作者将受到启发，建造下一代先进的成像仪器。

项目成果

Correlative microscopy reveals the nanoscale morphology of E. coli -derived supported lipid bilayers

相关显微镜揭示了大肠杆菌衍生的支持脂质双层的纳米级形态

• DOI: 10.1101/2021.11.04.467316
• 发表时间: 2021
• 作者: Bali K

Multiparametric sensing of outer membrane vesicle-derived supported lipid bilayers demonstrates the specificity of bacteriophage interactions

外膜囊泡衍生的支持的脂质双层的多参数传感证明了噬菌体相互作用的特异性

• DOI: 10.1101/2022.12.13.520201
• 发表时间: 2022
• 作者: Bali K

HSV-1 Glycoproteins Are Delivered to Virus Assembly Sites Through Dynamin-Dependent Endocytosis.

• DOI: 10.1111/tra.12340
• 发表时间: 2016-01
• 期刊: Traffic (Copenhagen, Denmark)
• 作者: Albecka A;Laine RF;Janssen AF;Kaminski CF;Crump CM
• 通讯作者: Crump CM

Multiparametric Sensing of Outer Membrane Vesicle-Derived Supported Lipid Bilayers Demonstrates the Specificity of Bacteriophage Interactions.

• DOI: 10.1021/acsbiomaterials.3c00021
• 发表时间: 2023-06-12
• 期刊: ACS BIOMATERIALS SCIENCE & ENGINEERING
• 影响因子: 5.8
• 作者: Bali, Karan;McCoy, Reece;Lu, Zixuan;Treiber, Jeremy;Savva, Achilleas;Kaminski, Clemens F.;Salmond, George;Salleo, Alberto;Mela, Ioanna;Monson, Rita;Owens, Roisin M.
• 通讯作者: Owens, Roisin M.

A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging.

• DOI: 10.1016/j.snb.2013.10.002
• 发表时间: 2014-03-01
• 期刊: Sensors and actuators. B, Chemical
• 作者: Bell L;Seshia A;Lando D;Laue E;Palayret M;Lee SF;Klenerman D
• 通讯作者: Klenerman D