Improved Microscopy Equipment for Undergraduate Microbiology

本科生微生物学改进的显微镜设备

• 批准号: 8950995
• 负责人: Thomas Kieft
• 金额: $ 1.75万
• 依托单位: New Mexico Institute of Mining and Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-04-15 至 1991-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8950995&HistoricalAwards=false
• 关键词: Improved; Microscopy; Equipment; Undergraduate; Microbiology

Modern microscopy techniques (phase contrast and epifluorescence microscopy) will enhance laboratory training and research in microbiology. Laboratory exercises will emphasize both techniques and application. Applications of phase contrast include identification of morphologically distinct groups of microorganisms in environmental samples, observations of microbial motility, and direct counts of microorganisms. Applications of epifluorescence microscopy include use of fluorescently labeled antibodies for identification and quantification of specific groups of microorganisms, and direct counts of microorganisms in environmental samples by acridene orange and fluorescein diacetate staining. The microscopy equipment and training gained by students will facilitate new experimentation in ongoing undergraduate research projects involving soil microbiology, chemolithotropic bacteria, and biological ice nucleation. The school will provide 50% matching funds.

现代显微镜技术（相差和落射荧光 显微镜）将加强实验室培训和研究， 微生物学. 实验室练习将强调技术和 应用程序. 相位对比的应用包括识别 环境中形态上不同的微生物类群 样品，微生物运动性的观察，以及 微生物的 落射荧光显微镜的应用包括使用 荧光标记抗体用于鉴定， 特定微生物组的定量和直接计数 吖啶橙子和 二乙酸荧光素染色。 显微镜设备和培训 学生获得的将促进正在进行的新实验 本科研究项目涉及土壤微生物学， 化能无机细菌和生物冰核。 学校将提供50%的配套资金。