Improved Microscopy Equipment for Undergraduate Microbiology

现代显微技术(相差显微镜和荧光显微镜)将加强微生物学方面的实验室培训和研究。实验练习将同时强调技术和应用。相位法的应用包括鉴定环境样品中形态上不同的微生物组，观察微生物的运动，以及微生物的直接计数。荧光显微镜的应用包括使用荧光标记的抗体来鉴定和定量特定的微生物组，以及通过丫啶橙和二醋酸荧光素染色直接计数环境样品中的微生物。学生获得的显微镜设备和培训将促进正在进行的涉及土壤微生物学、趋化性细菌和生物冰核的本科生研究项目中的新实验。学校将提供50%的配套资金。

Modern microscopy techniques (phase contrast and epifluorescence microscopy) will enhance laboratory training and research in microbiology. Laboratory exercises will emphasize both techniques and application. Applications of phase contrast include identification of morphologically distinct groups of microorganisms in environmental samples, observations of microbial motility, and direct counts of microorganisms. Applications of epifluorescence microscopy include use of fluorescently labeled antibodies for identification and quantification of specific groups of microorganisms, and direct counts of microorganisms in environmental samples by acridene orange and fluorescein diacetate staining. The microscopy equipment and training gained by students will facilitate new experimentation in ongoing undergraduate research projects involving soil microbiology, chemolithotropic bacteria, and biological ice nucleation. The school will provide 50% matching funds.