MRI: Acquisition of microscopy equipment to enhance histological research in Forensic Anthropology, Biology, and Bioarchaeology

MRI：购置显微镜设备以加强法医人类学、生物学和生物考古学的组织学研究

• 批准号: 1920218
• 负责人: Sophia Mavroudas
• 金额: $ 25.51万
• 依托单位: Texas State University - San Marcos
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1920218&HistoricalAwards=false
• 关键词: MRI; Acquisition; microscopy; equipment; enhance

With Major Research Instrumentation support, Sophia Mavroudas and her collaborators will purchase an integrated microscopy imaging and data analysis suite that consists of a Leica DM6M compound microscope and Leica M205C Stereoscope, both with internal cameras that are connected to a central workstation with an associated Leica Aperio ScanScope CS2 slide scanner. This will enhance Texas State University's research and outreach programs in forensic anthropology, biology, and bioarchaeology. This microscopy imaging suite is a user-friendly system that will impact the large group of students and researchers, from the undergraduate through to the postdoctoral level, who are part of the Forensic Anthropology Center at Texas State (FACTS) at Texas State University, a Hispanic Serving Institution (HSI). As an asset of FACTS, this equipment will foster synergistic collaborative research and interactive teaching. The addition of the microscopy suite will elevate the new PhD in Applied Anthropology Program and enhance faculty and student federally funded research in forensic anthropology, biology, and archaeology. The associated activities include the scientific training of women and underrepresented groups in histology and surface imaging, undergraduate STEM training, graduate student research and professionalization, and the expansion of FACTS workshops to include histology and surface imaging. The microscopy suite will allow for fast imaging of a variety of samples from 1x-1000x at one workstation which consists of the DM6M compound microscope, M205C stereosmicroscope, and Aperio ScopScan CS 2. This will be used to capture evidence from skeletal trauma, zooarchaeological samples, and artifact assemblages. Combined with existing FACTS resources, this equipment will support innovative experimental studies including controlled human decomposition experiments that can model the microscopic diagenesis of human bone throughout the post-mortem interval, as well as macroscopic trauma investigation from vulture scavenging. The associated stereoscope with imaging software will also allow for novel and detailed 3D modeling of skeletal trauma and pathology from documented collections as well as forensic casework and bioarchaeological samples. The acquisition of this equipment will help build and make available to researchers the first ever referential catalog of histological human variation from the rapidly expanding Texas State University Donated Skeletal Collection (TXSTDSC). The microscopy system will enable FACTS to build on existing research programs that focus on bone histology in various contexts including the investigation of human vs nonhuman bone differentiation, histological mammal species identification, human ageing, dental histology season-of-death estimation, dental histology studies of pathology and stress, the application of GIS technology to study bone histomorphology, and histological human variation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在重大研究仪器公司的支持下，Sophia Mavroudas和她的合作者将购买集成的显微镜成像和数据分析套件，该套件由徕卡DM6M复合显微镜和徕卡M205C立体镜组成，两者都带有内部摄像头，这些摄像头连接到中央工作站和相关的徕卡Aperio ScanScope CS2幻灯片扫描仪。这将加强德克萨斯州立大学在法医人类学、生物学和生物考古学方面的研究和推广项目。这个显微镜成像套件是一个用户友好的系统，将影响到大量的学生和研究人员，从本科生到博士后水平，他们是德克萨斯州立大学法医人类学中心(FACTS)的一部分，德克萨斯州立大学是一个拉美裔服务机构(HSI)。作为FACTS的一项资产，该设备将促进协同合作研究和互动教学。显微镜套件的增加将提升新的应用人类学博士学位，并加强教职员工和学生在法医人类学、生物学和考古学方面的联邦资助研究。相关活动包括对妇女和代表性不足群体进行组织学和表面成像方面的科学培训、本科生STEM培训、研究生研究和职业化，以及扩大FACTS讲习班，将组织学和表面成像包括在内。显微镜套件将允许在一个由DM6M复合显微镜、M205C立体显微镜和Aperio Scope CS 2组成的工作站上对1X-1000X的各种样本进行快速成像。这将用于从骨骼创伤、动物考古样本和文物组合中捕获证据。结合现有的FACTS资源，该设备将支持创新的实验研究，包括受控的人体分解实验，可以模拟人类骨骼在整个死后时间的微观成岩作用，以及秃鹫觅食造成的宏观创伤调查。与成像软件相关联的立体显微镜还将允许对骨骼创伤和病理进行新颖和详细的3D建模，这些模型来自有文件记录的收藏以及法医案件和生物考古样本。购买该设备将有助于建立并向研究人员提供从迅速扩大的德克萨斯州立大学捐赠的骨骼标本(TXSTDSC)中获得的首个组织人类变异参考目录。显微镜系统将使FACTS能够建立在现有研究项目的基础上，这些研究项目侧重于各种背景下的骨组织学，包括调查人类与非人类的骨分化、组织学哺乳动物物种鉴定、人类衰老、牙齿组织学死亡季节估计、牙齿组织学病理和压力研究、应用地理信息系统技术研究骨组织形态和组织人类变异。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。