Vertebrate Embryology/Developmental Biology Laboratory Improvement

该奖项为奥本大学动物与野生动物科学系脊椎动物胚胎学（ZY 302）实验室的升级提供资金。这笔资金将用于重新调整课程方向，使之朝着更“发展”的胚胎学方向发展。考虑基因活性和细胞分化的具体实验将被开发。光和紫外荧光显微镜将与不同蛇分枝和昆虫胚胎和幼虫阶段的解剖和固定组织一起使用。这些发育阶段将用适当的染色剂或荧光标记的抗体孵育，这样就可以证明基因激活和细胞分化。学生将获得最先进的显微镜设备和组织学和免疫荧光技术的第一手经验。他们的经验将包括考虑单克隆和多克隆抗体的发育免疫组织化学和讨论细胞机制参与分化。实验报告的特点是分析他们拍摄的实验结果，将加强学生对所考虑的发展现象的理解。本项目设备的选择基于质量、成本、耐用性以及与现有设备的兼容性。在与发育生物学相关的本科专题课程（ZY 498）中，该设备将有可能用于其他用途。受资助者正在匹配非联邦来源的奖励。

This award provides funds to upgrade the Vertebrate Embryology (ZY 302) laboratory in the Department of Zoology and Wildlife Sciences at Auburn University. The money will be use to re- orient the course toward a more "developmental" approach to the subject of embryology. Specific experiments that consider gene activity and the differentiation of cells will be developed. Light and UV-epifluorescence microscopy will be used in conjunction with dissected and fixed tissues from different opisthobranch and insect embryonic and larval stages. These developmental stages will have been incubated with appropriate stains or fluor-labeled antibodies such that gene activation and cell differentiation can be demonstrated. Students will obtain first-hand experience with state-of-the-art microscopical equipment and histological and immunofluorescent techniques. Their experience will include consideration of monoclonal versus polyclonal antibodies for developmental immunohistochemistry and discussion of cellular mechanisms involved in differentiation. Laboratory reports characterized by an analysis of their photographed experimental results will enhance the student's understanding of the developmental phenomena considered. Choices of equipment for this project have been based on quality, cost, durability, and compatibility with existing equipment. It will be possible for the equipment to serve additional use in undergraduate Special Topics courses (ZY 498) related to Developmental Biology. The grantee is matching the award from non-Federal sources.