Vertebrate Embryology/Developmental Biology Laboratory Improvement

脊椎动物胚胎学/发育生物学实验室改进

• 批准号: 8951912
• 负责人: Stephen Kempf
• 金额: $ 2.75万
• 依托单位: Auburn University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-02-01 至 1992-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8951912&HistoricalAwards=false
• 关键词: Vertebrate; Embryology; Developmental; Biology; Laboratory

This award provides funds to upgrade the Vertebrate Embryology (ZY 302) laboratory in the Department of Zoology and Wildlife Sciences at Auburn University. The money will be use to re- orient the course toward a more "developmental" approach to the subject of embryology. Specific experiments that consider gene activity and the differentiation of cells will be developed. Light and UV-epifluorescence microscopy will be used in conjunction with dissected and fixed tissues from different opisthobranch and insect embryonic and larval stages. These developmental stages will have been incubated with appropriate stains or fluor-labeled antibodies such that gene activation and cell differentiation can be demonstrated. Students will obtain first-hand experience with state-of-the-art microscopical equipment and histological and immunofluorescent techniques. Their experience will include consideration of monoclonal versus polyclonal antibodies for developmental immunohistochemistry and discussion of cellular mechanisms involved in differentiation. Laboratory reports characterized by an analysis of their photographed experimental results will enhance the student's understanding of the developmental phenomena considered. Choices of equipment for this project have been based on quality, cost, durability, and compatibility with existing equipment. It will be possible for the equipment to serve additional use in undergraduate Special Topics courses (ZY 498) related to Developmental Biology. The grantee is matching the award from non-Federal sources.

该奖项提供资金，以提升脊椎动物胚胎学 (ZY 302）动物学和野生动物系实验室 奥本大学的科学。 这笔钱将用于重新- 将课程定位于一种更“发展”的方法， 胚胎学的主题。 考虑基因的特定实验 细胞的活性和分化将得以发展。 光学和紫外荧光显微镜将用于 与来自不同组织的解剖和固定组织结合 后鳃和昆虫胚胎和幼虫阶段。 这些 发育阶段将与适当的 染色剂或荧光标记的抗体， 可以证明细胞分化。 学生将获得 最先进的显微镜的第一手经验 设备以及组织学和免疫荧光技术。 他们的经验将包括考虑单克隆与 用于发育免疫组织化学的多克隆抗体， 讨论分化中涉及的细胞机制。 实验室报告的特点是分析其 拍摄的实验结果将提高学生的 对所考虑的发展现象的理解。 选择 该项目的设备已根据质量，成本， 耐用性和与现有设备的兼容性。 它将 该设备可用于其他用途， 本科专题课程（ZY 498）相关 发育生物学。 受赠人正在匹配来自 非联邦来源