Embryology: Concepts & Techniques in Modern Developmental Biology

胚胎学：概念

• 批准号: 10392409
• 负责人: Linda E Hyman
• 金额: $ 16.03万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10392409
• 关键词: 3-Dimensional; Ablation; Address; Affect; Animals; Area; Behavior; Biological; Biology; Biomedical Research; CRISPR/Cas technology; Cell Lineage; Cell Therapy; Cells; Chickens; Child Health; Cnidaria; Collaborations; Complement; Congenital Abnormality; Course Content; Crustacea; Ctenophora; Development; Developmental Biology; Disease; Drosophila genus; Educational process of instructing; Electroporation; Embryo; Embryology; Environment; Evolution; Exposure to; Faculty; Future; Genetic; Goals; Human; Human Development; Imaging technology; Immersion; Influentials; Injury; International; Investigation; Laboratories; Light; Mentors; Methodology; Methods; Microscopy; Modeling; Modernization; Molecular; Molecular Genetics; Morphogenesis; Mus; Natural regeneration; Nematoda; Operative Surgical Procedures; Organism; Organogenesis; Participant; Pattern Formation; Planarians; Polishes; Postdoctoral Fellow; Process; RNA Interference; Rana; Reagent; Reporter; Research; Research Personnel; Research Training; Resolution; Scientific Advances and Accomplishments; Scientist; Sea Urchins; Series; Signal Transduction; Students; System; Talents; Techniques; Technology; Testing; Time; Tissue Grafts; Training; Transcriptional Regulation; Wood material; Work; Zebrafish; ascidian; base; career; comparative; confocal imaging; education research; educational atmosphere; exhaustion; experience; graduate student; hands on research; hemichordate; insight; instructor; instrumentation; laboratory experiment; lecturer; lectures; marine organism; microscopic imaging; next generation; novel; novel strategies; prevent; programs; regenerative biology; regenerative cell; repaired; stem cell biology; teaching assistant; tissue regeneration; tool; zygote

Program Summary The long term goal of the program in Embryology: Methods and Concepts in Modern Developmental Biology at the Marine Biological Laboratory (MBL) is to polish pre- and postdoctoral trainees into the leaders of research in developmental, regenerative, and stem cell biology. To accomplish this goal, the course comprehensively covers the paradigms, problems, and technologies of developmental biology cast within the comparative framework of animal evolution. The program is offered within the unique physical and intellectual environment of the summer at MBL, where hundreds of scientists and trainees come together to train in a variety of biomedically relevant fields, thereby providing ample opportunities for cross-disciplinary collaborations and discussions. Twenty-four students are selected each year from a very talented pool of diverse national and international applicants. The course curriculum involves a series of formal lectures, informal seminars, extended discussions, and an immersive laboratory focused on discovery research delivered over a six-week period in the summer. The teaching faculty are high-profile scientists in the field from across the US and around the world. Six modules, each one week long, are staffed by two full-time Course Directors, three to four faculty instructors, outside lecturers, and course and teaching assistants. The themes of the modules change from year-to-year as important advances in the field occur. Students are exposed to a wide variety of embryonic systems including well-established models, both genetic (fruit flies, nematodes, zebrafish, mouse) and experimental (chickens, sea urchins, frogs, ascidians). Students also encounter a range of non-traditional models such as marine organisms that exemplify animal diversity (e.g., cnidarians, nemerteans, planarians, crustaceans, molluscs, annelids, hemichordates, ctenophores). This broad coverage allows for a close examination of novel developmental strategies and mechanisms that may provide new insights into human related embryonic processes. Hands-on methodologies used to explore development involve surgical manipulation (cell ablation, tissue grafting) as well as molecular genetic tools (CRISPR/cas9, RNAi, electroporation) and cell biological approaches (use of genetic reporters to analyze cell lineage and migratory behavior). Students apply cutting-edge microscopy and imaging technologies (e.g., confocal imaging, light sheet microscopy, super-resolution microscopy and 3D time lapse) using state-of-the-art instrumentation, reagents, and methods. Conceptual topics include cell specification and differentiation, pattern formation, embryonic axis formation, morphogenesis, intercellular signaling, transcriptional regulation, organogenesis, regeneration, and comparative embryology. Students are challenged to formulate and test hypotheses, and work tirelessly in the well-equipped research and learning environment at the MBL. Alumni regularly state that this course was one of the most transformative and influential training experiences in their lives.

节目概要 胚胎学计划的长期目标：现代发育生物学的方法和概念 海洋生物实验室（MBL）旨在将博士前和博士后学员培养成研究领域的领导者 在发育、再生和干细胞生物学方面。为了实现这一目标，课程全面 涵盖了发育生物学的范式、问题和技术， 动物进化的框架。该计划是在独特的物理和智力环境中提供的 在MBL的夏季，数百名科学家和受训人员聚集在一起， 生物医学相关领域，从而为跨学科合作提供了充足的机会， 讨论。每年从一个非常有才华的不同国家和地区的学生中选出24名学生。 国际申请人。该课程包括一系列正式讲座，非正式研讨会， 扩展讨论，以及专注于发现研究的沉浸式实验室， 在夏天的时候。教师是来自美国各地的该领域的知名科学家， 世界各地六个模块，每个为期一周，由两名全职课程主任，三至四名 教师，外部讲师，课程和教学助理。模块的主题发生变化 随着该领域的重要进展，每年都有。学生接触到各种各样的 胚胎系统，包括成熟的模型，包括遗传模型（果蝇、线虫、斑马鱼、小鼠） 和实验动物（鸡、海胆、青蛙、海鞘）。学生们还遇到了一系列非传统的 诸如维持动物多样性的海洋生物的模型（例如，刺胞动物，纽形动物，真涡虫， 甲壳动物、软体动物、环节动物、半索动物、栉水母）。这种广泛的覆盖范围允许密切 研究新的发展战略和机制，可能提供新的见解，人类 相关的胚胎过程。用于探索开发的实践方法涉及外科手术 操作（细胞消融，组织移植）以及分子遗传工具（CRISPR/cas9，RNAi， 电穿孔）和细胞生物学方法（使用遗传报告基因分析细胞谱系和迁移 行为）。学生应用尖端的显微镜和成像技术（例如，共焦成像，光 薄片显微镜、超分辨率显微镜和3D延时摄影），使用最先进的仪器， 试剂和方法。概念性主题包括细胞规格和分化、模式形成， 胚轴形成，形态发生，细胞间信号传导，转录调控，器官发生， 再生和比较胚胎学。学生面临的挑战是制定和测试假设， 在MBL设备齐全的研究和学习环境中不知疲倦地工作。校友们经常说， 该课程是他们一生中最具变革性和影响力的培训经历之一。