Integrating and Updating Biochemistry/Molecular Biology Experiments

整合和更新生物化学/分子生物学实验

• 批准号: 8951966
• 负责人: Rodney Boyer
• 金额: $ 2.01万
• 依托单位: Hope College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 1992-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=8951966&HistoricalAwards=false
• 关键词: Integrating; Updating; Biochemistry; Molecular; Biology

A new integrated laboratory course for biochemistry/molecular biology majors will combine contents of the existing biochemistry course with a newly developed molecular biology component. The first half of the course will focus on isolation and characterization of biomolecules. The second half will concentrate on techniques in molecular biology and gene manipulation. New equipment including column chromatography, a high speed refreigerated centrifuge, a refrigerated laboratory cabinet, gel electrophoresis equipment, microcentrifuge, incubator- shaker, and bacteriological incubator will be used for experiments in mutagenesis techniques, mutant analysis, bacteriophage purification, transposons, bacterial conjugation, plasmid isolation, restriction endonuclease digestion, DNA cloning, and transformation. The college will provide 50% matching funds.

生物化学/分子生物学专业的新综合实验室课程将现有生物化学课程的内容与新开发的分子生物学部分相结合。课程的前半部分将重点关注生物分子的分离和表征。 下半场将集中于分子生物学和基因操作技术。新设备包括柱层析、高速冷冻离心机、冷冻实验室柜、凝胶电泳设备、微量离心机、培养摇床、细菌培养箱等，将用于诱变技术、突变体分析、噬菌体纯化、转座子、细菌接合、质粒分离、限制性内切酶消化等实验。 DNA 克隆和转化。 学院将提供50%的配套资金。