Metabolism and Ion Transport in the Arctic Amphipod Gammarus oceanicus

北极片脚类 Gammarus oceanicus 的代谢和离子传输

• 批准号: 9000608
• 负责人: Joseph Torres
• 金额: $ 3.65万
• 依托单位: University of South Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-06-01 至 1991-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9000608&HistoricalAwards=false
• 关键词: Metabolism; Ion; Transport; Arctic; Amphipod

Gammarid amphipods form an important trophic link between algae and meiofauna, and fishes, birds, and seals in high Arctic latitude environments. Their success may be attributed to physiological mechanisms that allow them to flourish under extreme cold temperatures and changing salinities induced by freezing and melting sea ice. The proposed research focuses on extraordinarily abundant Arctic amphipod that lives in the intertidal zone and under sea ice. Energy cost of osmoregulation will be examined by direct measurement of oxygen consumption in a graded series of salinities and temperatures. Physiological measurements will be complemented by determinations of activity of ion-transport protein: Na-K ATPase. In addition, the ultrastructure of gill membranes will be examined for changes in quantity of ion transport sites and structure as a function of salinity and temperature. The project will be done in collaboration with scientists from the University of Trondheim, and in cooperation with the Norwegian Polar Institute for Arctic Research. Norwegian scientists will be conducting parallel experiments on the response of internal sodium, potassium, chloride and free amino acids to changing salinity and temperature.

在北极高纬度环境中，双足类动物在藻类、小型动物、鱼类、鸟类和海豹之间形成了重要的营养联系。它们的成功可能归因于生理机制，使它们能够在极端寒冷的温度和由海冰冻结和融化引起的盐度变化下蓬勃发展。拟议中的研究重点是生活在潮间带和海冰下的极其丰富的北极片脚类动物。渗透调节的能量成本将通过直接测量在盐度和温度梯度下的耗氧量来检验。生理测量将补充离子转运蛋白的活性测定：Na-K atp酶。此外，鳃膜的超微结构将被检查离子转运位点的数量和结构的变化作为盐度和温度的函数。该项目将与特隆赫姆大学的科学家合作完成，并与挪威极地研究所合作进行北极研究。挪威科学家将对内部钠、钾、氯化物和游离氨基酸对盐度和温度变化的反应进行平行实验。