Structure and Function of a Novel Biotin Enzyme

本研究的重点是理解的意义和 生物素酶在植物代谢中的作用。 实现这一 目的建立胡萝卜独特的体细胞胚胎发生体系， 被用来研究这些蛋白质。 期间 胡萝卜体细胞胚的稳定发育 六种含生物素多肽之一的水平 急剧增加。 这种生物素蛋白已经被 纯化的多克隆抗体已经被 制备 使用这些抗体，编码以下的cDNA克隆 这种生物素蛋白已经被分离出来。 这种新型生物素 蛋白质正在研究通过利用 这些抗体和cDNA克隆作为特异性探针， 研究这种新型生物素蛋白。 这些调查 着重阐明了 这种生物素酶。 抗体的初步实验 已经鉴定出这种生物素蛋白代表乙酰基 辅酶A羧化酶。 这种形式的乙酰辅酶A的功能 羧化酶在植物代谢中的作用通过以下方式阐明：1） 确定这种细胞的亚细胞和组织分布， 胡萝卜植物中的蛋白质，以及2）阐明胡萝卜植物中的蛋白质的分子结构。 调节这种积累的遗传机制 蛋白质对发育和环境 信号. 生物素是一组酶的重要组成部分， 具有重要的代谢功能。 结构、功能 许多生物素酶的调节广泛地 在一组不同的生物体中进行研究。 然而， 对植物的生物素酶是已知的。 本研究 研究植物生物素的功能和调节， 为了更好地理解 在植物代谢中的作用 *//

This research focuses on understanding the significance and role of biotin enzymes in plant metabolism. To achieve this goal the unique somatic embryogenesis system of carrot has been utilized to study these proteins. During the development of somatic embryos of carrot the steady state levels of one of the six biotin-containing polypeptides increases dramatically. This biotin protein has been purified and polyclonal antibodies against it have been prepared. Using these antibodies a cDNA clone coding for this biotin protein has been isolated. This novel biotin protein is being investigated through the utilization of these antibodies and the cDNA clone as specific probes to investigate this novel biotin protein. These investigations concentrate on elucidating the function and regulation of this biotin enzyme. Initial experiments with the antibodies have identified this biotin protein as representing an acetyl CoA carboxylase. The function of this form of acetyl-CoA carboxylase in plant metabolism is being elucidated by 1) identifying the subcellular and tissue distribution of this protein in the carrot plant and 2) elucidating the molecular genetic mechanisms that regulate the accumulation of this protein in response to developmental and environmental signals. Biotin is an essential component of a set of enzymes that have important metabolic functions. The structure, function and regulation of a number of biotin enzymes is extensively studied in a diverse set of organisms. However, very little is known about the biotin enzymes of plants. This research investigates the function and regulation of plant biotin- containing enzymes in order to provide a better understanding of their role in plant metabolism.***//