MRI: Acquisition of a high resolution Accela-LTQ FT Ultra mass spectrometer system

这一重大研究仪器(MRI)奖用于资助爱荷华州立大学WM Keck代谢组学研究实验室购买Bruker-Daltonics SolariXPrivate FTMS Bundle质谱仪系统，并支持整合化学和生物化学、生物科学和工程学科的多个系的多学科研究。新的仪器系统将为研究人员提供前所未有的生物分子数据，从而加强大学和国家的研究基础设施。新仪器系统支持的项目试图应对在代谢水平上破译对基因组表达的功能理解的挑战，并且是对特定代谢和基因网络的结构和调节的假设驱动的研究和寻求产生关于基因功能的假设的高通量全球图谱项目的混合体。这些研究项目的影响力将大大增强SolariXPrivate FTMS束质谱计系统，因为研究人员将能够更严格地确定代谢物的化学特性，从而生成和测试关于基因产品和基因网络的代谢功能的更可靠的假设。此外，该仪器将为教师提供一个以研究为基础的多学科培训工具，学生将获得如何进行现代代谢研究的洞察力，整合化学和基因组生物学的进步。来自爱荷华州立大学和邻近大学的博士后、研究生、本科生，以及来自爱荷华州的高中生和教师也将参与研究。这些外展活动将通过暑期研讨会、实习计划和NSF-REU计划进行协调，这些计划通过中心(例如，CBiRC)和将利用该工具的项目(例如，拟南芥2010代谢组学联盟项目)已经到位。因此，学生将得到广泛的指导和培训，以迎接21世纪新出现的技术挑战。研究项目的结果将通过学生和教职员工在区域或国家会议上的陈述以及同行评议文献中的出版物来传播。

This Major Research Instrumentation (MRI) award funds the acquisition of a Bruker-Daltonics SolariXHybrid FTMS Bundle mass spectrometer system at the Iowa State University WM Keck Metabolomics Research Laboratory, and empowers multidisciplinary research from a wide sweep of departments that integrate disciplines of chemistry and biochemistry, biological sciences and engineering. The new instrument system will provide researchers with unprecedented data concerning biological molecules and thus enhance the research infrastructure of the University and the State. The projects supported by the new instrument system attempt to meet the challenge of deciphering functional understanding of genome expression at the metabolic level, and are a mixture of hypothesis-driven research on the structure and regulation of specific metabolic and gene networks, and high-throughput global profiling projects that seek to generate hypotheses concerning gene functionalities. The impact of these research projects will be greatly enhanced the SolariXHybrid FTMS Bundle mass spectrometer system because researchers will be able to more rigorously determine the chemical identities of metabolites and hence generate and test more robust hypotheses concerning the metabolic functionalities of gene products and gene networks.In addition, this instrument will provide faculty a vehicle for the multi-disciplinary research-based training of students, who will gain insights into how to conduct modern metabolic studies, integrating advances in chemistry and genomics biology. Post-docs, graduate students, undergraduate students from Iowa State University and neighboring colleges, and high-school students and teachers from the State of Iowa will also participate in research. These latter outreach activities will be coordinated through summer workshops, internship programs and NSF-REU programs that are already in place through the Centers (e.g., CBiRC) and projects (e.g., The Arabidopsis 2010 Metabolomics Consortium Project) that will utilize the instrument. Therefore students will be broadly mentored and trained to meet the emerging technological challenges of the 21st century. Results from the research projects will be disseminated through student and faculty presentations at regional or national meetings, and through publications in the peer-reviewed literature.