MRI: Acquisition of a high resolution Accela-LTQ FT Ultra mass spectrometer system

该重大研究仪器（MRI）奖项为爱荷华州州立大学WM Keck代谢组学研究实验室收购Bruker-Daltonics SolariXHybrid FTMS Bundle质谱仪系统提供资金，并支持来自广泛部门的多学科研究，这些部门整合了化学和生物化学，生物科学和工程学科。新的仪器系统将为研究人员提供有关生物分子的前所未有的数据，从而加强大学和国家的研究基础设施。由新仪器系统支持的项目试图应对在代谢水平上破译基因组表达的功能理解的挑战，并且是对特定代谢和基因网络的结构和调节的假设驱动的研究和寻求产生关于基因功能的假设的高通量全球分析项目的混合物。SolariXHybrid FTMS Bundle质谱仪系统将大大增强这些研究项目的影响力，因为研究人员将能够更严格地确定代谢物的化学身份，从而生成和测试有关基因产物和基因网络代谢功能的更可靠的假设。此外，该仪器将为教师提供多学科研究为基础的学生培训工具，他们将深入了解如何进行现代代谢研究，整合化学和基因组学生物学的进步。来自爱荷华州州立大学和邻近学院的博士后、研究生、本科生以及来自爱荷华州的高中生和教师也将参加研究。这些后一种外联活动将通过暑期讲习班、实习方案和已经通过中心实施的NSF-REU方案进行协调（例如，CBiRC）和项目（例如，拟南芥2010代谢组学联盟项目）将利用该仪器。因此，学生将得到广泛的指导和培训，以满足21世纪世纪新兴的技术挑战。研究项目的结果将通过学生和教师在区域或国家会议上的演讲以及通过同行评审文献中的出版物进行传播。

This Major Research Instrumentation (MRI) award funds the acquisition of a Bruker-Daltonics SolariXHybrid FTMS Bundle mass spectrometer system at the Iowa State University WM Keck Metabolomics Research Laboratory, and empowers multidisciplinary research from a wide sweep of departments that integrate disciplines of chemistry and biochemistry, biological sciences and engineering. The new instrument system will provide researchers with unprecedented data concerning biological molecules and thus enhance the research infrastructure of the University and the State. The projects supported by the new instrument system attempt to meet the challenge of deciphering functional understanding of genome expression at the metabolic level, and are a mixture of hypothesis-driven research on the structure and regulation of specific metabolic and gene networks, and high-throughput global profiling projects that seek to generate hypotheses concerning gene functionalities. The impact of these research projects will be greatly enhanced the SolariXHybrid FTMS Bundle mass spectrometer system because researchers will be able to more rigorously determine the chemical identities of metabolites and hence generate and test more robust hypotheses concerning the metabolic functionalities of gene products and gene networks.In addition, this instrument will provide faculty a vehicle for the multi-disciplinary research-based training of students, who will gain insights into how to conduct modern metabolic studies, integrating advances in chemistry and genomics biology. Post-docs, graduate students, undergraduate students from Iowa State University and neighboring colleges, and high-school students and teachers from the State of Iowa will also participate in research. These latter outreach activities will be coordinated through summer workshops, internship programs and NSF-REU programs that are already in place through the Centers (e.g., CBiRC) and projects (e.g., The Arabidopsis 2010 Metabolomics Consortium Project) that will utilize the instrument. Therefore students will be broadly mentored and trained to meet the emerging technological challenges of the 21st century. Results from the research projects will be disseminated through student and faculty presentations at regional or national meetings, and through publications in the peer-reviewed literature.