Molecular Mechanisms of Sodium Channel Sorting in Neurons

神经元钠通道排序的分子机制

• 批准号: 9006436
• 负责人: William Gilly
• 金额: $ 1.67万
• 依托单位: Stanford University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-01 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9006436&HistoricalAwards=false
• 关键词: Molecular; Mechanisms; Sodium; Channel; Sorting

Nerve cell activity depends on various ions crossing the cell membrane through channels that are sometimes very specific. These channels are formed by protein molecules in the membrane. Nerve cells often have a long process, the axon which extends from the cell body known as the soma. Often channels in the axon are distributed differently from those in the soma. This research addresses the question of how different proteins, which are continuously synthesized in the soma of nerve cells, are spatially distributed. This project will examine the molecular mechanisms of sorting sodium channel proteins in nerve cells. Sodium channel proteins are synthesized in the soma, but are found in the membrane of the axon and are absent in the soma. In the squid, an invertebrate, mRNA coding from rat sodium channels will be injected into the soma. As a result the cells will synthesize two different types of sodium channels making it possible to compare the spatial distribution of the two protein molecules. The part of the molecule responsible for its peculiar spatial distribution can be changed by changing the coding and thus is able to be identified. This study will advance our understanding of long-term regulation of how nerve cells function.

神经细胞的活动取决于穿过细胞膜的各种离子，这些离子有时是非常特定的。 这些通道是由膜中的蛋白质分子形成的。 神经细胞通常有一个很长的过程，从细胞体延伸出来的轴突被称为胞体。 通常，轴突中的通道与体细胞中的通道分布不同。 这项研究解决了神经细胞体中不断合成的不同蛋白质如何在空间上分布的问题。 该项目将研究神经细胞中钠通道蛋白分类的分子机制。 钠通道蛋白在体细胞中合成，但存在于轴突膜中，并且在体细胞中不存在。在鱿鱼中，来自大鼠钠通道的无脊椎动物编码 mRNA 将被注射到体细胞中。 结果，细胞将合成两种不同类型的钠通道，从而可以比较两种蛋白质分子的空间分布。 分子中负责其特殊空间分布的部分可以通过改变编码来改变，从而能够被识别。 这项研究将增进我们对神经细胞功能的长期调节的理解。