Small Grants for Exploratory Research (SGER): Preparative- Scale Isoelectric Focusing in Open Channels Using Thermally-Engendered pH Gradients

探索性研究小额资助 (SGER)：使用热产生 pH 梯度在开放通道中进行制备级等电聚焦

• 批准号: 9006538
• 负责人: Charles Lochmuller
• 金额: $ 5万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-07-15 至 1991-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9006538&HistoricalAwards=false
• 关键词: Small; Grants; Exploratory; Research; SGER

This grant is in the areas of engineering and analytical chemistry and in the subarea of chromatography. Professor Lochmuller will study the design and application of a continuous, open-channel, isoelectric focusing system that will separate ampholytes of broad size scale using thermally produced pH gradients. This research will aim to develop both batch and continuous isoelectric focusing systems whose separating capabilities will arise from thermally produced pH gradients. The long- term impact of this research will be in the development of simple, preparative isoelectric focusing instruments, as opposed to existing analytical instruments, that are capable of resolving and efficiently separating both large proteins and whole cells.

该补助金用于工程和分析领域 化学和色谱的子领域。 教授 Lochmuller将研究一种 连续、开放通道、等电聚焦系统， 将使用热分离宽尺寸尺度的两性电解质 产生pH梯度。 本研究旨在开发批量和连续 等电聚焦系统，其分离能力 将由热产生的pH梯度引起。 很长的- 这项研究的长期影响将是在发展 简单的，制备等电聚焦仪器，如 与现有的分析仪器相反， 将两种大的蛋白质 整个细胞。