Laser Ablation Time-of-Flight Mass Spectrometry of DNA

DNA 激光烧蚀飞行时间质谱分析

• 批准号: 9006939
• 负责人: Peter Williams
• 金额: $ 5万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-03-15 至 1991-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9006939&HistoricalAwards=false
• 关键词: Laser; Ablation; Time; Flight; Mass

This Grant for Exploratory Research is in the general area of analytical and surface chemistry and in the subfield of mass spectrometry. Professor Peter Williams of Arizona State University will study the production of molecular ion species from laser volatilization of frozen aqueous matrices of DNA. The goal is to minimize the fragmentation of the DNA and preserve the molecular weight distribution of the biological molecule. The development of a suitable means to use mass spectrometry to sequence biological molecules will result. Via the use of a novel energy deposition mechanism, both the absorptivity and the phase transformation thermodynamics of the matrix are used to preserve structure. The construction of a high resolution flight tube for time of flight mass spectrometry will allow immediate understanding of the role of laser energy deposition parameters on the minimization of fragmentation.

这项探索性研究拨款是在分析和表面化学的一般领域，以及在质谱学的子领域。亚利桑那州立大学的彼得·威廉姆斯教授将研究激光挥发冻结的DNA水基质产生分子离子物种。目标是最大限度地减少DNA的碎裂，并保持生物分子的分子量分布。开发一种合适的方法来使用质谱学来对生物分子进行排序将是结果。通过一种新的能量沉积机制，利用基质的吸收率和相变热力学来保持结构不变。用于飞行时间质谱仪的高分辨率飞行管的建造将使人们能够立即了解激光能量沉积参数在最大限度地减少碎裂方面的作用。