RUI: Nuclear Cytology and Centrin in the Red Algae

RUI：红藻中的核细胞学和中心蛋白

• 批准号: 9008078
• 负责人: Joseph Scott
• 金额: $ 22.73万
• 依托单位: College of William and Mary
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9008078&HistoricalAwards=false
• 关键词: RUI; Nuclear; Cytology; Centrin; Red

Drs. Joseph Scott and Sharon Broadwater of the College of William and Mary are studying the details of cell division in groups of red algae (Rhodophyta) using scanning and transmisson electron microscopy. The number, kinds, and orientations of subcellular structures combine to create patterns of cell division that appear to be characteristic for major groups of red algae at the ordinal or subclass taxonomic level. Research is continuing on cell division patterns in groups of Corallinales and Batrachospermales, which at present are poorly known. The investigators are also conducting a search in red algae for the recently described protein centrin, which is a contractile protein thought to be present in all eukaryotic cells and involved in chromosome movement. In addition, the enigmatic unicellular organism Glaucosphaera vacuolata is being studied through electron microscopy and molecular and immunocytochemical methods. One of the unique characters of Rhodophyta, alone among the world's algae, is the lack of a flagellar apparatus, yet Drs. Scott and Broadwater have preliminary data indicating that G. vacuolata possesses a non-emergent flagellum located within a red-algal cytoplasm. Molecular and immunocytochemical tests are being made with the help of collaborators, to confirm the presence of centrin and of a reduced flagellum. The results will provide new insights into phylogenetic relationships within the red algae and with other groups of primitive organisms.

约瑟夫·斯科特博士和莎伦·布罗德沃特博士， 威廉和玛丽正在研究细胞分裂的细节， 利用扫描和透射技术对红藻（Rhodophyta）类群的研究 电镜 的数量、种类和方向 亚细胞结构联合收割机形成细胞图案 似乎是红色主要群体的特征 藻类在序数或亚类分类水平上。 研究是 继续研究珊瑚目的细胞分裂模式 和Batrachospermales，目前知之甚少。 调查人员还在红藻中进行搜索 对于最近描述的蛋白质中心蛋白，这是一个 一种被认为存在于所有真核细胞中的收缩蛋白 并参与染色体运动。 此外，神秘的 单细胞生物Glaucosphaera vacuolata 通过电子显微镜、分子和免疫细胞化学 方法. 红藻门的独特特征之一， 世界上的藻类，是缺乏鞭毛机构，但博士。 斯科特和布罗德沃特的初步数据表明，G。 vacuolata拥有一个位于 红藻细胞质 分子和免疫细胞化学检测是 在合作者的帮助下， 存在中心蛋白和减少的鞭毛。 结果将 提供了新的见解系统发育关系内 红藻和其他原始生物群。