Long & Medium Term Research: Evolutionary Genetics of Host Plant Choice in a Willow Leaf Beetle

长期中期研究：宿主的进化遗传学 杨柳叶甲的植物选择。 该奖项是根据长期计划提出的。 &在国外卓越中心的中期研究。 的 该计划旨在使美国科学家和工程师能够 在国外的研究机构进行长期研究， 证明卓越。 奖项提供了机会， 开展联合研究，以及使用独特或复杂的 实验设施、专业知识和实验条件 国外 该奖项将支持内森博士为期12个月的访问 排名比利时和瑞士，与博士。 Jacques Pasteels，Universit> Libre de Bruxelles，and Dr. Martine Rowell-Rafel，大学动物学研究所 巴塞尔，关于“进化遗传学的寄主植物选择， 杨柳叶甲虫。“为了更好地了解 昆虫寄主转移的进化后果， 首席研究员建议完成一项研究， 宿主利用的区域差异与 杨柳叶甲遗传分化研究 （鞘翅目：叶甲科），常见于整个欧亚大陆，其中 在不同的地区有不同的宿主 一般的杨柳甲虫 人口结构高度细分， 甚至在很小的空间尺度上的遗传分化。 以来 卵黄疫霉的嗜食性存在区域差异， 细分人口结构，它将提供一个优秀的 本研究的模型。 在他以前关于 北美杨柳叶甲的种群结构 和Rowell-Raiden教授对区域变化的研究， 卵黄疫霉的寄主植物利用，首席研究员 我会问：1）昆虫如何进化出对新宿主的偏好 植物种类？2）昆虫是否形成遗传上的寄生虫- 当他们完成宿主转换的时候， 到 回答这些问题，他将：1）使用电泳标记， 测量P. vitellinae的种群结构，并确定 无论种群是沿着寄主物种分化的 2）进行宿主植物的遗传分析 喜好 实验方案将包括1） 群体内遗传变异的量化 2）确定遗传方式; 通过交叉种群的偏好， 偏好的变化。 因为首席调查员 将使用相同的P. vitellinae种群进行电穿孔， 通过血液学研究和基因分析，他将能够 将宿主使用的变化直接与 遗传分化

Long & Medium-Term Research: Evolutionary Genetics of Host Plant Choice in a Willow Leaf Beetle. This award recommendation is made under the Program for Long & Medium-Term Research at Foreign Centers of Excellence. The program seeks to enable U.S. scientists and engineers to conduct long-term research abroad at research institutions of proven excellence. Awards provide opportunities for the conduct of joint research, and the use of unique or compli- mentary facilities, expertise and experimental conditions in foreign countries. This award will support a twelve-month visit by Dr. Nathan Rank to both Belgium and Switzerland, to work with Dr. Jacques Pasteels, Universit> Libre de Bruxelles, and Dr. Martine Rowell-Rahier, Zoologisches Institut der Universitaet Basel, on "Evolutionary Genetics of Host Plant Choice in a Willow Leaf Beetle." In order better to understand the evolutionary consequences of host shifts in insects, the principal investigator proposes to complete a study of the relationship between regional variation in host use and genetic divergence in a willow leaf beetle, Phratora vitellinae (Coleoptera: Chrysomelidae), common throughout Eurasia, which is known to vary hosts regionally. Willow beetles in general have a highly subdivided population structure, with measurable genetic differentiation at even small spatial scales. Since P. vitellinae has the regional variation in preference and a subdivision population structure, it will provide an excellent model for this study. Building on his previous work on the population structure of a North American willow leaf beetle and Prof. Rowell-Rahier's research on regional variation in P. vitellinae's host plant use, the principal investigator will ask: 1) How do insects evolve preferences for new host plant species?, and 2) Do insects form genetically differen- tiated host races when they accomplish a host shift? To answer these, he will: 1) Use electrophoretic markers to measure P. vitellinae's population structure and to determine whether populations are differentiated along host species lines; and 2) Conduct a genetic analysis of host plant preferences. The experimental protocol will include 1) quantification of genetic variation within populations in preference and 2) determination of the mode of inheritance of preference by crossing populations which show regional variation in preference. Because the principal investigator will use the same P. vitellinae populations for the electrop- horetic study and the genetic analysis, he will be able to relate variations in host use directly with patterns of genetic differentiation.