Instrumentation for DNA and Protein Characterization

DNA 和蛋白质表征仪器

• 批准号: 9009948
• 负责人: Henry Nguyen
• 金额: $ 13.63万
• 依托单位: Texas Tech University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1992-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9009948&HistoricalAwards=false
• 关键词: Instrumentation; DNA; Protein; Characterization

The research to be carried out by the ten co-principal investigators involves experimental techniques drawn from molecular biology and protein biochemistry to study: (1) protein structure/function relationships; (2) the ability of plants to synthesize cellulose and to respond to temperature stress and (3) evolutionary relationships in reptiles and mammals. The requested instrumentation, which will constitute the core biological facility, will provide a central, shared group of modern instruments that will enable the co-P.I.'s and other research faculty in the three co-operating academic departments represented in this proposal; to isolate proteins on a large scale; to purify proteins, peptides and oligonucleotides to homogeneity; to isolate, purify and amplify DNA samples; to obtain amino acid resolution of proteins; to analyze electrophoresis results quickly and with high DNA and to carry out site-directed mutagenesis.

研究将由十位共同负责人进行 研究人员涉及从分子水平提取的实验技术， 生物学和蛋白质生物化学研究：（1）蛋白质 结构/功能关系;（2）植物的能力， 合成纤维素并响应温度胁迫和（3） 爬行动物和哺乳动物的进化关系。 请求的 仪器，这将构成核心生物 设施，将提供一个中央，共享组的现代 这些工具将使co-P.I.和其他研究 三个合作学术部门的教师代表 在这个提议中，大规模分离蛋白质，纯化 蛋白质、肽和寡核苷酸至同质;分离， 纯化和扩增DNA样品;获得氨基酸分辨率 蛋白质;快速分析电泳结果， DNA并进行定点突变。