Instrumentation for DNA and Protein Characterization

十位共同首席研究员将进行的研究涉及从分子生物学和蛋白质生物化学中提取的实验技术，以研究：(1)蛋白质结构/功能关系；(2)植物合成纤维素和对温度胁迫的反应能力；(3)爬行动物和哺乳动物的进化关系。所要求的仪器将构成核心生物设施，将提供一个中央、共享的现代仪器组，使合作项目成为可能。本提案中所代表的三个合作学术部门的研究人员；分离：大规模地分离蛋白质；纯化蛋白质、多肽和寡核苷酸至均匀性；分离、纯化和扩增DNA样本；获得蛋白质的氨基酸分辨率；快速、高DNA分析电泳结果，进行定点诱变。

The research to be carried out by the ten co-principal investigators involves experimental techniques drawn from molecular biology and protein biochemistry to study: (1) protein structure/function relationships; (2) the ability of plants to synthesize cellulose and to respond to temperature stress and (3) evolutionary relationships in reptiles and mammals. The requested instrumentation, which will constitute the core biological facility, will provide a central, shared group of modern instruments that will enable the co-P.I.'s and other research faculty in the three co-operating academic departments represented in this proposal; to isolate proteins on a large scale; to purify proteins, peptides and oligonucleotides to homogeneity; to isolate, purify and amplify DNA samples; to obtain amino acid resolution of proteins; to analyze electrophoresis results quickly and with high DNA and to carry out site-directed mutagenesis.