Search for Enzymes with Long Recognition Sequences, U.S.- Pakistan Collaboration

项目描述：本项目为冷泉港实验室的Richard Roberts博士和巴基斯坦拉合尔旁遮普省大学高级分子生物学中心的Sheikh Riazuddin博士继续开展限制性内切酶的合作研究提供支持。这两位科学家已经合作了大约两年，分离微生物，寻找新的II型酶的存在，并确定它们的序列特异性。该项目将使这项研究得以继续进行，以便对来自巴基斯坦不同地方环境的微生物菌株进行分离和分类鉴定；筛选II型限制性内切酶菌株，特别注意具有长识别序列的酶；以及新的限制性内切酶的纯化和表征，以及那些具有新的特异性的酶，以及对切割位点的识别。范围：该项目符合发展中国家科学项目关于美国和巴基斯坦科学家参与研究的标准。它允许来自巴基斯坦的年轻科学家在美国最好的分子生物学研究实验室之一接受培训，并将该领域的现代技术应用于该国正在进行的研究。它还允许在巴基斯坦建立一个限制性内切酶储存库，这对分子生物学研究至关重要。这种能力对巴基斯坦的基础和应用基因工程研究非常重要，也为美国的研究增加了可用酶的多样性

Description: This project provides support for Dr Richard Roberts of Cold Spring Harbor Laboratory and Dr. Sheikh Riazuddin of the Center for Advanced Molecular Biology, University of Punjab, Lahore, Pakistan, to continue collaborative research on restriction endonucleases. The two scientists have been collaborating for about two years on isolating microbes and searching for the presence of new Type II enzymes, and determining their sequence specificity. This project will allow the continuation of this research for isolation and taxonomical characterization of microbial strains from different local environment in Pakistan; screening of bacterial strains for the presence of Type II restriction endonucleases with special attention to enzymes with long recognition sequences; and the purification and characterization of the new restriction enzymes, and those with new specificities, with recognition of the site of cleavage. Scope: This project meets the criteria of the Science in Developmening Countries Program criteria for research participation by the U.S. and Pakistani scientists. It allows for training of young scientists from Pakistan in one of the best U.S. laboratories in molecular biology research, and the application of modern techniques in that field to research being done in that country. It also allows for creating a repository, in Pakistan, of restriction enzymes which are essential for molecular biology research. That capability is important for research in basic and applied genetic engineering in Pakistan, and it also provides for increasing the diversity in the available enzymes for research in the U.S.