Search for Enzymes with Long Recognition Sequences, U.S.- Pakistan Collaboration

描述：该项目为理查德博士提供支持 冷泉港实验室的罗伯茨和谢赫博士 高级分子生物学中心的Riazuddin说， 巴基斯坦拉合尔旁遮普大学，继续 限制性内切酶的合作研究。 的 两位科学家已经合作了两年 分离微生物并寻找新的 II型酶，并确定其序列特异性。 该项目将使这项研究得以继续 用于微生物的分离和分类鉴定 来自巴基斯坦不同地方环境的菌株; 筛选细菌菌株以确定是否存在II型 特别关注酶的限制性内切核酸酶 具有长识别序列;并且纯化和 新限制性内切酶的特性，以及那些 具有新的特异性， 乳沟 范围：本项目符合《科学》标准， 发展中国家方案研究标准 美国和巴基斯坦科学家的参与。 它 允许来自巴基斯坦的年轻科学家在一个 美国最好的分子生物学实验室 以及现代技术在这一领域的应用， 在这个国家进行的研究。 它还允许 在巴基斯坦建立了一个限制性内切酶的仓库 这是分子生物学研究所必需的。 的 能力对于基础研究和应用研究都很重要 基因工程在巴基斯坦，它也提供了 增加可用酶的多样性， 在美国的研究

Description: This project provides support for Dr Richard Roberts of Cold Spring Harbor Laboratory and Dr. Sheikh Riazuddin of the Center for Advanced Molecular Biology, University of Punjab, Lahore, Pakistan, to continue collaborative research on restriction endonucleases. The two scientists have been collaborating for about two years on isolating microbes and searching for the presence of new Type II enzymes, and determining their sequence specificity. This project will allow the continuation of this research for isolation and taxonomical characterization of microbial strains from different local environment in Pakistan; screening of bacterial strains for the presence of Type II restriction endonucleases with special attention to enzymes with long recognition sequences; and the purification and characterization of the new restriction enzymes, and those with new specificities, with recognition of the site of cleavage. Scope: This project meets the criteria of the Science in Developmening Countries Program criteria for research participation by the U.S. and Pakistani scientists. It allows for training of young scientists from Pakistan in one of the best U.S. laboratories in molecular biology research, and the application of modern techniques in that field to research being done in that country. It also allows for creating a repository, in Pakistan, of restriction enzymes which are essential for molecular biology research. That capability is important for research in basic and applied genetic engineering in Pakistan, and it also provides for increasing the diversity in the available enzymes for research in the U.S.