Study of Restriction Endonucleases

目标和相关性：本行动支持纽约长岛冷泉港实验室的Richard J. Roberts博士和巴基斯坦拉合尔旁遮普大学高级分子生物学中心的Sheikh Riazuddin博士之间的合作研究。这项研究将涉及生产各种具有新特异性的新型限制性内切酶，用于基因工程。将注意那些允许发现识别序列长于6个核苷酸的酶的方法，因为这种不经常切割DNA的酶对绘制大型基因组（如人类基因组）具有重要价值。美巴项目支持两国科学家的合作研究，因为两国的能力可以互补。这个项目很好地满足了这一标准。优点：两位科学家都是各自国家分子生物学领域最受尊敬的科学家。他们过去曾合作进行科学研究，并在国际期刊上发表了联合文章。这个项目很可能产生可发表的结果，这将使在该领域工作的其他人受益。资助：本项目获两笔拨款资助；旁遮普邦大学，资助INT-8713479: FY87: SFC$22,925（巴基斯坦货币）冷泉港实验室，资助INT-8713519: FY87: us $20,925加SFC$5,000 FY88: us $22,181

Objective and Relevancy: This action supports collaborative research between Dr. Richard J. Roberts of Cold Spring Harbor Laboratory in Long Island, NY and Dr. Sheikh Riazuddin of the Center of Advanced Molecular Biology at the University of Punjab, Lahore, Pakistan. The research will involve production of a wide variety of new restriction enzymes with new specificities for use in genetic engineering. Attention will be paid to methods that allow the discovery of enzymes with recognition sequences longer than 6 nucleotides, since such enzymes which cleave DNA infrequently are of great value for mapping large genomes such as human genomes. The U.S.-Pakistan program supports cooperative research involving scientists from the two countries where capabilities complement each other. This project meets that criterion well. Merit: The two scientists are among the most highly regarded in the field of molecular biology in their respective countries. They have collaborated on scientific research in the past with results published as joint articles in international journals. This project is likely to produce publishable results which would benefit others working in the field. Funding: The project is being funded under two grants; University of Punjab, grant INT-8713479: FY87: SFC$22,925 (Pakistani Currency) Cold Spring Harbor Laboratory, grant INT-8713519: FY87: U.S.$20,925 plus SFC$5,000 FY88: U.S.$22,181