Molecular Analysis of Brassinolide Action in Plant Growth and Development

油菜素内酯在植物生长发育中作用的分子分析

• 批准号: 9013409
• 负责人: Steven Clouse
• 金额: $ 2.1万
• 依托单位: San Diego State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-05-01 至 1991-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9013409&HistoricalAwards=false
• 关键词: Molecular; Analysis; Brassinolide; Action; Plant

Dr. Clouse is requesting funds to conduct a one year pilotprogram on the effects of a novel plant growth steroid,brassinolide (BR), on gene expression during development. He hason hand the chemically pure compound, a set of molecular probesand transgenic plants and three assay systems with which toobtain, within one year, definitive answers about thefeasibility of establishing a long.term program on the action ofthis steroid compound on the transcription of plant genesinvolved in growth and differentiation. No work on the action ofthis compound on gene transcription has been reported and thelack of preliminary molecular data make this unsuitable for afull scale proposal. BR has pronounced effects on plant growthand development, interacts with other plant growth regulators andshares structural similarities with animal steroid hormones. Ithas immense potential as a tool for probing gene expressionduring development and as an agricultural growth promoter. %%% Nothing is known about the mechanism of action of brassinolide. This pilot study will determine if it has a proximal effect ongene expression. If so then a larger research effort would bewarranted.

克劳斯博士正在申请资金进行为期一年的试点项目 关于一种新的植物生长类固醇，油菜素（BR）， 基因表达的影响。他已经把 化学纯化合物，一套分子探针和转基因 植物和三个测定系统，以获得，在一个 一年，关于建立一个 长期计划的行动ofthis类固醇化合物对 参与植物生长的基因转录， 分化没有关于该化合物对基因的作用的研究 转录已被报道，缺乏初步的 分子数据使其不适合于全规模的提议。BR 对植物的生长发育有显著的影响， 与其他植物生长调节剂， 与动物类固醇激素相似。它是巨大的 作为探测发育过程中基因表达的工具 和作为农业生长促进剂。 %%% 目前尚不清楚的是， 油菜籽这项试点研究将确定它是否具有 基因表达的邻近效应如果是这样的话， 努力将是值得的。