Arabidopsis 2010: Protein Interacting Networks and Site-Specific Phosphorylation in Leucine-Rich Repeat Receptor-Like Kinase Function

拟南芥 2010：富含亮氨酸的重复受体样激酶功能中的蛋白质相互作用网络和位点特异性磷酸化

• 批准号: 1021363
• 负责人: Steven Clouse
• 金额: $ 250.41万
• 依托单位: North Carolina State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-01 至 2015-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1021363&HistoricalAwards=false
• 关键词: Arabidopsis; 2010; Protein; Interacting; Networks

Plant and animal cells sense and respond to signals in their environment through receptors that relay biochemical information via protein-protein interactions to the nucleus, where changes in gene expression occur. One class of these receptors (the Leucine-Rich Repeat Receptor-like kinases or LRR RLKs) is present in a large family of 223 members in the model plant Arabidopsis thaliana. These receptors have a structural organization similar to that of animal receptor kinases, and several that have been studied in detail are known to be critical elements that regulate the plant's growth, morphogenesis, disease resistance, and responses to stress. However, the functions of the vast majority of this large family of signal transduction molecules remain unknown. With previous support from the NSF Arabidopsis 2010 Program, the investigators have generated a set of genetic and biochemical resources that will allow the in-depth study of the function of LRR RLKs in plant growth and stress responses. Using proteomic and mass spectrometry approaches, this project is designed to define the proteins that interact with these LRR RLKs. These proteins form an interactive network whose function ultimately determines a range of physiological responses, and this project will define the components and properties of this network. In addition to this broad study of protein network interactions, a selected group of LRR-RLKs will be characterized at a detailed biochemical level, including functional analyses that will permit an understanding of how these proteins influence plant growth.Broader Impact of the Research: Given the known importance of the LRR RLK family to plant development, the results of this project are likely to have practical agricultural impact. In addition this project will provide extensive resources to the Arabidopsis research community including a database (http://www4.ncsu.edu/~sclouse/Clouse2010.htm) that creates a unique resource for the comparative study of LRR RLK protein networks and phosphorylation sites. The highly successful NSF-supported Plant Phosphorylation Workshop will be reinitiated and will serve as a forum for researchers from as many as 45 laboratories to interact and share research ideas and results. The project will also provide training in protein biochemistry, mass spectrometry, and Arabidopsis molecular genetics at all levels from high school student interns through postdoctoral scientists. Recruitment of these individuals will be integrated with established programs at North Carolina State University and the University of Illinois that encourage the participation of under-represented minorities in scientific research.

植物和动物细胞通过受体感知和响应环境中的信号，这些受体通过蛋白质-蛋白质相互作用将生化信息传递到细胞核，在细胞核中发生基因表达的变化。这些受体中的一类（富含亮氨酸重复序列的受体样激酶或LRR RLK）存在于模式植物拟南芥（Arabidopsis thaliana）中的223个成员的大家族中。这些受体具有与动物受体激酶类似的结构组织，并且已经详细研究的几种已知是调节植物生长、形态发生、抗病性和对胁迫的反应的关键元件。然而，这个信号转导分子大家族中绝大多数的功能仍然未知。在NSF Arabidopsis 2010计划的支持下，研究人员已经产生了一组遗传和生化资源，这些资源将允许深入研究LRR RLKs在植物生长和胁迫反应中的功能。使用蛋白质组学和质谱方法，该项目旨在确定与这些LRR RLKs相互作用的蛋白质。这些蛋白质形成了一个相互作用的网络，其功能最终决定了一系列的生理反应，该项目将定义该网络的组件和属性。除了这个广泛的研究蛋白质网络的相互作用，一组选定的LRR-RLKs将在一个详细的生化水平，包括功能分析，将允许了解这些蛋白质如何影响植物growth.Broader影响的研究：鉴于已知的重要性的LRR RLK家庭植物发育，该项目的结果很可能有实际的农业影响。此外，该项目还将为拟南芥研究社区提供广泛的资源，包括一个数据库（http：//www4.ncsu.edu/clousclouse/Clouse2010.htm），该数据库为LRR RLK蛋白网络和磷酸化位点的比较研究创建了一个独特的资源。非常成功的NSF支持的植物磷酸化研讨会将重新启动，并将作为一个论坛，来自多达45个实验室的研究人员互动和分享研究思路和成果。该项目还将提供从高中实习生到博士后科学家的各级蛋白质生物化学，质谱和拟南芥分子遗传学方面的培训。这些人的招募将与北卡罗来纳州州立大学和伊利诺伊大学的既定计划相结合，这些计划鼓励代表性不足的少数民族参与科学研究。