U.S.-UK Cooperative Research: Regulation of the Plasma Membrane and Tonoplast H+-ATPases in Plants Exposed to NaC1

该奖项将支持博士之间的合作研究。 保罗·长谷川，普渡大学园艺系， T博士J. Flowers和A. R.生物学院Yeo School of Biological 科学，苏塞克斯大学，英格兰。 的目标 拟议的项目是研究等离子体的反应， 膜和液泡膜ATPase对盐胁迫的响应 盐度是限制农业生产的一个主要因素， 因为它降低了耕地的产量。盐 植物的耐受性涉及到整合各种机制， 细胞学和解剖学组织的各个层次。 至关重要的机制是那些发挥作用的机制， 调节离子积累的速率， 高离子积累的影响，但促进充分 生长所必需的渗透调节的溶质摄取 新的渗透环境有理由设想 质膜和液泡膜起着核心作用 调节溶质积累和区室化。 然而，实验结果并没有定义物理- 质膜的生理或生物化学作用， 液泡膜。 该项目将侧重于 质膜和液泡膜H+转运ATP酶 因为它们在H+的产生中起着核心作用， 电化学梯度穿过相应的膜， 介导促进跨膜溶质输入到 cell. 这个项目的目的是提供证据 关于这些H+泵在 调节溶质输入到工厂期间的制度， 盐税 该项目将受益于 美国和英国调查人员的互补专业知识 在盐胁迫分子生物学和生理学中的应用。 的 这项研究的结果可能具有重要的经济意义。 对农业的影响。

This award will support collaborative research between Dr. Paul Hasegawa, Department of Horticulture, Purdue University, and Drs. T. J. Flowers and A. R. Yeo, School of Biological Sciences, University of Sussex, England. The objective of the proposed project is to study the response of the plasma membrane and tonoplast ATPase to salt stress. Salinity is a major factor limiting agricultural production, since it reduces yields in land under cultivation. Salt tolerance in plants involves the integration of mechanisms at all levels of cytological and anatomical organization. Mechanisms of critical importance are those which function to regulate the rate of ion accumulation and mediate the effects of high ion accumulation, yet promote sufficient solute uptake for osmotic adjustment necessary for growth in the new osmotic environment. It is reasonable to envisage that the plasma membrane and the tonoplast have central roles in regulated solute accumulation and compartmentation. However, experimental results have not defined the physi- ological or biochemical roles of the plasma membrane and tonoplast in plasma membrane. This project will focus on the plasma membrane and tonoplast H+ - translocating ATPases because of their central roles in the generation of the H+ electrochemical gradient across the respective membranes, which mediates facilitated transmembrane solute import into the cell. The objective of this project is to provide evidence regarding the significance of these H+ - pumps in the regulation of solute import into the plant during regimes of salt imposition. This project will benefit from the complementary expertise of the US and British investigators in molecular biology and physiology of salt stress. The results of this research may have important economic implications for agriculture.