Biogenesis of Peroxisomes in Plants

植物中过氧化物酶体的生物发生

• 批准号: 9018088
• 负责人: Richard Trelease
• 金额: $ 18.6万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-15 至 1993-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9018088&HistoricalAwards=false
• 关键词: Biogenesis; Peroxisomes; Plants

Three different aspects of plant peroxisome biogenesis will be pursued: (1), the means by which the expanding membrane of oilseed glyoxysomes acquires its protein and lipid is an integral part of the organelle biogenesis. The hypothesis will be tested that lipid bodies contribute membrane lipid to differentiating oilseed glyoxysomes; (2), parameters and conditions necessary and/or sufficient for recognition and tranlocation in vivo of malate synthase, isocitrate lyase and catalase into peroxisomes of cultured monkey kidney and selected plant cells will be determined; (3) studies will continue on deciphering patterns of regulation and developmental expression of catalase isoforms during the changeover from glyoxysomes to leaf-type peroxisomes, and of isocitrate lyase and malate synthase during glyoxysome differentiation in maturing and germinated seeds. The availability of an embryo culture system is expected to provide a unique means to help resolve regulation patterns within maturing seeds. Peroxisomes occur in virtually all eukaryotic cells, conducting a variety of essential metabolic functions depending on the cell type in which they reside. They are not capable of synthesizing their own phospholipids or proteins, and are therefore dependent upon the selective and complicated intracellular trafficking mechanisms responsible for their biogenesis. The objectives of this proposal are to examine the molecular and biochemical bases of selected, interrelated aspects of peroxisome biogenesis; the emphasis will be on cottonseed glyoxysomes.

植物过氧化物酶体生物发生的三个不同方面将被 （1）、膜的扩张方式 油籽乙醛酸酶体获得其蛋白质和脂质是一个整体 细胞器生物发生的一部分。 假设将被检验 脂质体有助于膜脂分化， 油籽乙醛酸酶体;（2），所需参数和条件 和/或足以在体内识别和转运 苹果酸合酶、异柠檬酸裂解酶和过氧化氢酶转化为过氧化物酶体 培养的猴子肾脏和选定的植物细胞将被 （3）研究将继续破译模式， 过氧化氢酶异构体的调节和发育表达 在从乙醛酸酶体到叶型过氧化物酶体的转变过程中， 和异柠檬酸裂合酶和苹果酸合成酶在乙醛酸体 分化成熟和发芽的种子。 的 胚胎培养系统的可用性预计将提供 独特的手段，以帮助解决监管模式， 种子 过氧化物酶体存在于几乎所有的真核细胞中， 各种基本代谢功能取决于细胞 他们居住的类型。 它们无法合成 它们自身的磷脂或蛋白质， 选择性和复杂的细胞内运输 负责其生物起源的机制。 的目标 这项建议是要检查分子和生物化学基础， 过氧化物酶体生物发生的选定的、相互关联的方面; 重点将放在棉籽乙二醛酶体上。