Characterization of the Homophilic Adhesion of the Myelin Protein Po

髓鞘蛋白 Po 的同质粘附的表征

• 批准号: 9021615
• 负责人: Marie Filbin
• 金额: $ 22.9万
• 依托单位: CUNY Hunter College
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1994-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9021615&HistoricalAwards=false
• 关键词: Characterization; Homophilic; Adhesion; Myelin; Protein

Myelin, a substance formed by Schwann cells in the nervous system, provides an insulating barrier around nerve fibers. During neuronal maturation, myelin is formed by the compaction of membrane sheets and requires the precise organization of a number of specific proteins. The long-term goals of this research are to understand how membranes of myelin are held together at the molecular level. The immediate goal is to investigate the Po protein, a major constituent of myelin that has an essential role in the maintenance of myelin shealth compactness. The Po protein contains adhesion domains that allow Po molecules to attach to each other. Dr. Filbin will characterize the Po-Po interaction by determining what manipulations of the Po molecule affect increase adhesion in transfected Chinese Hamster Ovary cells expressing Po protein. Experiments using Po-peptides and Po antibodies will be performed to determine the amino acid sequences essential for adhesion. Results from this study will help elucidate how Po functions in myelin.

髓磷脂，一种由神经系统中的许旺细胞形成的物质， 系统，在神经纤维周围提供绝缘屏障。 在神经元成熟过程中，髓磷脂是由神经元的致密化形成的。 薄膜片，需要精确的组织， 特定的蛋白质。 这项研究的长期目标是 为了了解髓磷脂膜是如何在 分子水平。 当前的目标是调查阿宝 蛋白质，髓鞘的主要成分，具有重要作用， 维持髓鞘的致密性。 Po蛋白 含有粘附结构域，使Po分子能够附着在 对方. 菲尔宾博士将描述Po-Po相互作用 通过确定Po分子的操作会影响 转染中国人卵巢癌细胞粘附性增加 表达Po蛋白。 使用Po-肽和Po的实验 将进行抗体检测以确定氨基酸 粘合所必需的序列。 这项研究的结果将 有助于阐明Po在髓磷脂中的功能