SGER: Are Viruses a Significant Component of Dissolved DNA Marine Environment
SGER: Are Viruses a Significant Component of Dissolved DNA Marine Environment
批准号:
9022036
负责人:
John Paul
金额:
$4.12万
依托单位国家:
美国
项目类别:
Standard Grant
财政年份:
1990
资助国家:
美国
项目状态:
已结题
起止时间:
1990-10-15 至 1992-09-30
中文摘要
海洋环境中溶解的DNA的存在已经有了充分的记录。最近,病毒已被证明是海水溶解部分的重要生物成分。目前尚不清楚溶解的DNA中有多少是真正可溶的DNA,多少是包裹在病毒颗粒内的DNA。环境中可溶性DNA和完整病毒粒子的分化和定量对生态学和遗传学都具有重要意义。从生态学的角度来看,可溶性DNA是大分子生物合成的一种核酸前体。毫无疑问,病毒有助于溶解DNA和有机碳的产生,并通过裂解宿主来控制微生物种群。保罗博士将开发从水柱中的游离DNA中分离和定量病毒颗粒的方法。本研究将为今后的研究提供快速分离病毒的方法。
英文摘要
The existence of dissolved DNA in the marine environment has been well documented. Recently, viruses have been shown to be an important biological component of the dissolved fraction of seawater.It is not known what proportion of the dissolved DNA is truly soluble DNA or DNA encapsulated within a virus particle. Differentiation and quantitation of the soluble DNA and the intact virions in the environment is important for both ecological and genetic reasons. From the ecological perspective, soluble DNA is a form of nucleic acid precursors for macromolecular biosynthesis. Viruses no doubt contribute to dissolved DNA and organic carbon production and control the microbial population by lysing their hosts. Dr. Paul will develop methods to separate and quantitate viral particles from free DNA in the water column. This study will lead to methodology for the rapid isolation of viruses for future study.
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Gene Transfer Agents in the Oceans-What are they doing?
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批准号:0801593
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项目类别:Standard Grant
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资助金额:$50.0万
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财政年份:2008
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负责人:John Paul
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依托单位:
BE/IDEA: An Autonomous Genosensor for Environmental Water Quality Monitoring
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批准号:0216305
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项目类别:Standard Grant
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资助金额:$128.96万
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财政年份:2002
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负责人:John Paul
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依托单位:
BE/GEN-EN: Marine Viromics: The Interaction of Viral Genomes with the Marine Environment
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批准号:0221763
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项目类别:Standard Grant
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资助金额:$192.81万
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财政年份:2002
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负责人:John Paul
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依托单位:
The Paradox of the Viroplankton: High Viral Abundance and Resistance to Infection
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批准号:9811319
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项目类别:Standard Grant
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资助金额:$26.23万
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财政年份:1998
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负责人:John Paul
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依托单位:
Instrumentation for Molecular Analysis of Marine Organisms
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批准号:9512122
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项目类别:Standard Grant
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资助金额:$10.04万
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财政年份:1995
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负责人:John Paul
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依托单位:
Viral - Bacterial Interactions in the Marine Environment: Significance of Lysogeny
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批准号:9502775
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项目类别:Continuing Grant
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资助金额:$30.0万
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财政年份:1995
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负责人:John Paul
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依托单位:
Molecular Regulation of Carbon Fixation in Oceanic Picoplankton
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批准号:9216937
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项目类别:Continuing Grant
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资助金额:$18.4万
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财政年份:1992
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负责人:John Paul
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依托单位:
Acquisition of a Transmission Electron Microscope and Elemental Microanalysis Science Building of the Univ. of South Florida.
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批准号:9214614
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项目类别:Standard Grant
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资助金额:$15.0万
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财政年份:1992
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负责人:John Paul
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依托单位:
The Genetic Significance of Viruses in the Marine Environment
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批准号:9115942
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项目类别:Continuing Grant
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资助金额:$31.83万
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财政年份:1992
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负责人:John Paul
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依托单位:
REU: Bacterial Transformation as a Mechanism of Gene Transfer in the Marine Environment
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批准号:8817172
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项目类别:Continuing Grant
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资助金额:$18.72万
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财政年份:1989
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负责人:John Paul
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依托单位:
The Cycling of Extracellular DNA in Oligotrophic and Eutrophic Rivers of Southwest Florida
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批准号:8605170
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项目类别:Continuing Grant
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资助金额:$28.44万
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财政年份:1986
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负责人:John Paul
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依托单位:
Acquisition of Equipment for the University of South Florida's Department of Marine Science
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批准号:8507343
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项目类别:Standard Grant
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资助金额:$5.77万
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财政年份:1985
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负责人:John Paul
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依托单位:
The Dynamics of dissolved DNA in Planktonic Environments
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批准号:8415605
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项目类别:Standard Grant
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资助金额:$9.4万
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财政年份:1985
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负责人:John Paul
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依托单位:
Maximization of Agar Biosynthesis in the Red Algae
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批准号:8360570
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项目类别:Standard Grant
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资助金额:$3.36万
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财政年份:1984
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负责人:John Paul
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依托单位:
Equipment For the Department of Marine Science, University Of South Florida
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批准号:8307366
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项目类别:Standard Grant
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资助金额:$4.5万
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财政年份:1983
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负责人:John Paul
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依托单位:
海外基金