权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Regulation of Carbon Fixation in Oceanic Picoplankton

海洋超微型浮游生物固碳的分子调控

基本信息

批准号：
9216937
负责人：
John Paul
金额：
$ 18.4万
依托单位：
University of South Florida
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
1992
资助国家：
美国
起止时间：
1992-11-15 至 1996-10-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=9216937&HistoricalAwards=false
关键词：
Molecular Regulation Carbon Fixation Oceanic

项目摘要

Ribulose bisphosphate carboxylase-oxygenase (RuBPCase) is the enzyme responsible for nearly all carbon fixation on this planet and is the major carboxylating enzyme in the phytoplankton of the world's oceans. Additionally, RuBPCase may play a global role in modulating one of the greenhouse gases, CO2. Knowledge of the regulation of this enzyme is important for understanding control of primary production in the oceans and phytoplankton response to environmental perturbations or long term change. Dr. Paul and his collaborator, Dr. Tabita, will focus on the molecular regulation of this enzyme in picoplankton in coastal and oligotrophic oceanic environments. They have recently developed a method for extraction of mRNA from phytoplankton and have detected RuBPCase gene expression in natural populations. These biomolecular techniques will be used to study diel and light-dependent regulation of the RuBPCase gene in cultured Synechococcus spp. and prochlorophytes, and in natural populations. The second objective is to identify and characterize the key primary producers by amplifying RUBPCase mRNA for cloning and sequencing from natural populations. Amplification of mRNA will result in clones that originated from transcriptionally active phototrophs. Sequence data will be compared to RuBPCase data bases to identify the active species. If novel phototrophs are encountered, parsimony analysis will be performed to relate these forms to their evolutionary closest relative. In summary, the project will provide fundamental information on the control of the RuBPCase genes in response to environmental factors and identify key phototrophic organisms in oceanic picoplankton.

二磷酸核酮糖羧化酶-加氧酶（RuBPCase）是负责地球上几乎所有碳固定的酶是浮游植物的主要羧化酶，世界的海洋。此外，RuBPCase可能在以下方面发挥全球性作用：调节一种温室气体二氧化碳。知识这种酶的调节对于理解海洋初级生产力和浮游植物对环境变化或长期变化。保罗博士和他的合作者Tabita博士将专注于分子调控，这种酶在沿海和贫营养海洋中的微型浮游生物中环境. 他们最近发明了一种提取从浮游植物中检测到RuBPCase基因在自然人群中的表达。这些生物分子技术将被用于研究昼夜和光依赖性调节，聚球藻RuBPCase基因的研究和前绿藻，在自然人群中。第二个目标是确定并通过扩增RUBPCase来表征主要初级生产者用于从自然群体中克隆和测序的mRNA。 mRNA的扩增将导致源自于转录活跃的光养生物。序列数据将与RuBPCase数据库进行比较以鉴定活性物质。如果遇到新的光养生物，简约分析将是将这些形式与它们进化上最接近的亲戚总之，该项目将提供基本的关于RuBPCase基因响应于环境因素并确定关键的光养生物海洋微型浮游生物