Faculty Awards for Women

女性教师奖

• 批准号: 9024174
• 负责人: Nien-Hwa Wang
• 金额: $ 25万
• 依托单位: Purdue Research Foundation
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-11-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9024174&HistoricalAwards=false
• 关键词: Faculty; Awards; Women

There are two thrusts to this research. First, fundamental studies to characterize the intrinsic kinetics and adsorption parameters of two key pharmaceutical, insulin and urokinase, in ion exchange, hydrophobic interaction, and affinity chromatography systems will be made. Measurements of the equilibrium isotherms and kinetic constants of the pure compounds will be made and the effects of pH and salt level on the affinities and kinetic constants will be investigated. An existing dynamic column simulation model will be extended to accommodate gradient systems. The model will be validated with pure component experimental data and then will be used to study how impurities and products compete for sorbent sites. As a second and supporting thrust the complex interplay between biomolecules and sorbent functional groups resulting from steric effects, hydration, as well as hydrophobic and electrostatic interactions will be studied. This research should provide a complete description of the configurations of a complex during binding, allow quantitative assessment of various intermolecular forces, and predict the reactive affinities for different molecules. In this way, the understanding of how molecular structure affects sorbent selectivity will be advanced. The research will also have a significant impact on the synthesis and identification of more selective sorbents for biochemical separations.

这项研究有两个重点。 第一，基础研究 表征本征动力学和吸附参数 胰岛素和尿激酶这两种关键药物的离子交换， 疏水相互作用，亲和层析系统将 被制造。 平衡等温线和动力学的测量 纯化合物的常数将被确定，pH值的影响 和盐的水平上的亲和力和动力学常数将 研究了 现有的动态柱模拟模型将 延伸以适应梯度系统。 该车型将 用纯组分实验数据进行验证，然后将 用于研究杂质和产品如何竞争吸附剂 网站. 作为第二个和支持推力， 生物分子和吸附剂官能团之间的相互作用， 空间效应、水合作用以及疏水和静电 将研究相互作用。 这项研究将提供一个 完整描述了一个复杂的配置， 结合，允许定量评估各种分子间 力，并预测不同的反应亲和力 分子。 通过这种方式， 结构影响吸附剂选择性的研究将进一步深入。 的 研究也将对综合和 生物化学吸附剂选择性鉴定 分居