Faculty Awards for Women

这项研究有两个重点。首先，将对胰岛素和尿激酶这两种关键药物在离子交换、疏水相互作用和亲和色谱体系中的内在动力学和吸附参数进行基础研究。测量了纯化合物的平衡等温线和动力学常数，并研究了pH和盐水平对亲和常数和动力学常数的影响。现有的动态柱模拟模型将被扩展以适应梯度系统。该模型将用纯组分实验数据进行验证，然后将用于研究杂质和产品如何竞争吸附位点。作为第二个和支持推力，生物分子和吸收官能团之间的复杂相互作用，由空间效应，水合作用，以及疏水和静电相互作用将被研究。这项研究应该提供一个完整的描述在结合过程中的复合物的构型，允许定量评估各种分子间的力，并预测不同分子的反应亲和。通过这种方式，对分子结构如何影响吸附剂选择性的理解将得到推进。该研究还将对生化分离中更多选择性吸附剂的合成和鉴定产生重大影响。

There are two thrusts to this research. First, fundamental studies to characterize the intrinsic kinetics and adsorption parameters of two key pharmaceutical, insulin and urokinase, in ion exchange, hydrophobic interaction, and affinity chromatography systems will be made. Measurements of the equilibrium isotherms and kinetic constants of the pure compounds will be made and the effects of pH and salt level on the affinities and kinetic constants will be investigated. An existing dynamic column simulation model will be extended to accommodate gradient systems. The model will be validated with pure component experimental data and then will be used to study how impurities and products compete for sorbent sites. As a second and supporting thrust the complex interplay between biomolecules and sorbent functional groups resulting from steric effects, hydration, as well as hydrophobic and electrostatic interactions will be studied. This research should provide a complete description of the configurations of a complex during binding, allow quantitative assessment of various intermolecular forces, and predict the reactive affinities for different molecules. In this way, the understanding of how molecular structure affects sorbent selectivity will be advanced. The research will also have a significant impact on the synthesis and identification of more selective sorbents for biochemical separations.