Faculty Awards for Women

这项研究有两个突破口。首先，我们将对离子交换、疏水作用和亲和层析体系中两种关键药物--胰岛素和尿激酶的本征动力学和吸附参数进行基础性研究。测定了纯化合物的平衡等温线和动力学常数，考察了pH和盐度对亲和力和动力学常数的影响。现有的动态柱模拟模型将扩展以适应梯度系统。该模型将用纯成分实验数据进行验证，然后将用于研究杂质和产品如何竞争吸附剂位置。作为第二个和支撑性的推力，我们将研究生物分子和吸附官能团之间由于空间效应、水合作用以及疏水和静电相互作用而产生的复杂相互作用。这项研究应该提供一个络合物在结合过程中的构型的完整描述，允许对各种分子间作用力进行定量评估，并预测不同分子的反应亲和力。这样，对分子结构如何影响脱硫剂选择性的理解将会更深入。这项研究还将对合成和鉴定用于生化分离的更具选择性的吸附剂产生重大影响。

There are two thrusts to this research. First, fundamental studies to characterize the intrinsic kinetics and adsorption parameters of two key pharmaceutical, insulin and urokinase, in ion exchange, hydrophobic interaction, and affinity chromatography systems will be made. Measurements of the equilibrium isotherms and kinetic constants of the pure compounds will be made and the effects of pH and salt level on the affinities and kinetic constants will be investigated. An existing dynamic column simulation model will be extended to accommodate gradient systems. The model will be validated with pure component experimental data and then will be used to study how impurities and products compete for sorbent sites. As a second and supporting thrust the complex interplay between biomolecules and sorbent functional groups resulting from steric effects, hydration, as well as hydrophobic and electrostatic interactions will be studied. This research should provide a complete description of the configurations of a complex during binding, allow quantitative assessment of various intermolecular forces, and predict the reactive affinities for different molecules. In this way, the understanding of how molecular structure affects sorbent selectivity will be advanced. The research will also have a significant impact on the synthesis and identification of more selective sorbents for biochemical separations.