Rapid Kinetics Using Stopped-Flow and UV/Visible Absorption Spectroscopy

使用停流和紫外/可见吸收光谱的快速动力学

• 批准号: 9050453
• 负责人: James Larrabee
• 金额: $ 1.17万
• 依托单位: Middlebury College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-15 至 1992-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9050453&HistoricalAwards=false
• 关键词: Rapid; Kinetics; Using; Stopped; Flow

A stopped-flow (SF) rapid mixing accessary is being used to introduce undergraduate chemistry, biochemistry and molecular biology students to an important method for studying fast reactions. The SF accessory is primarily used with a UV/Visible absorption spectrometer (UV/VIS), computerized and modified for rapid data collection. Reactions with half-lives on the order of 20-50 milliseconds are studied with the system. Many biological enzyme-catalyzed reactions are studied with the SF/UV/VIS system. The SF/UV/VIS instrument is used by junior majors in a combined analytical, inorganic and physical chemistry laboratory. Senior thesis research students use the SF/UV/VIS instrument to study the oxygenation of cobalt-substituted hemocyanin, The hands-on experience with a technique used to study rapid reaction has enhanced the students' understanding of concepts of biochemical and chemical kinetics. The institution has contributed to this project in an amount equal to the NSF funds.

一种停止流动（SF）快速混合辅助装置被用来向本科化学、生物化学和分子生物学的学生介绍一种研究快速反应的重要方法。SF配件主要用于紫外/可见吸收光谱仪（UV/VIS），计算机化和修改，以快速收集数据。用该系统研究了半衰期为20-50毫秒的反应。利用SF/UV/VIS系统研究了许多生物酶催化反应。SF/UV/VIS仪器供大三学生在分析、无机、物理化学综合实验室使用。毕业论文研究生利用SF/UV/VIS仪器研究钴取代血青素的氧合作用。通过对快速反应技术的实际操作，提高了学生对生化和化学动力学概念的理解。该机构已为该项目提供了相当于国家科学基金会基金的金额。