Integration of Specialized Light Microscopy into Undergrad- uate Biology Education

荧光显微镜、相位对比度和差分干涉对比度(DIC)光学和高分辨率视频成像的结合彻底改变了我们对细胞生物学的理解，特别是在细胞运动和细胞骨架、细胞内运输、细胞器行为、细胞内信号和蛋白质动力学等领域。这些技术正在通过购买组合相位、DIC和荧光显微镜、用于现有Brightfield显微镜的5个相位对比度转换套件、高分辨率视频系统和倒置相位对比度显微镜而被整合到课程中。荧光显微镜将与免疫荧光染色结合使用，用于蛋白质的亚细胞定位，以及用于膜和细胞器的荧光探针染色。倒立位相对比和DIC显微镜将用于对活细胞的观察。视频系统将允许对细胞事件进行集体观察和讨论。该设备将用于学生研究项目以及细胞生物学课程。学院将捐赠相当于该奖项的金额。

The combination of epifluorescence microscopy, phase contrast and differential interference contrast (DIC) optics, and high resolution video imaging have revolutionized our understanding of cell biology; particularly in the areas of cell motility and the cytoskeleton, intracellular trafficking, cellular organelle behavior, intracellular signaling and protein dynamics. These techniques are being integrated into the curriculum through the purchase of a combination phase, DIC, and epifluorescence microscope, 5 phase contrast conversion kits for existing brightfield microscopes, a high resolution video system, and an inverted phase contrast microscope. Fluorescence microscopy will be used in conjunction with immunofluorescent staining for the subcellular localization of proteins, and fluorescent probe staining for membranes and organelles. Inverted and upright phase contrast and DIC microscopy will be used for observations on living cells. The video system will allow for group observation and discussion of cellular events. The equipment will be used in student research projects as well as the Cell Biology course. The college will contribute an amount equal to the award.