Species Specific Division Rates of Diatoms: Response to Changes in Dissolved Silicate Concentrations

在不同的生长条件下， 自然种群是最受欢迎的测量方法之一 在浮游植物生态学中。 这项研究计划将 研究硅藻生长速率对扰动的响应 在溶解的硅酸盐供应中使用荧光染料（罗丹明 123）。 在罗丹明123中孵育的硅藻积极积累它 进入二氧化硅沉积囊泡，罗丹明123是共- 用二氧化硅沉积。 由此产生的瓣膜和腰带带 成为荧光，并提供细胞分裂的标记， 硅藻;从而允许估计物种特异性 增长率。 将进行实验以研究 低溶解硅酸盐浓度限制 吸收溶解的硅酸盐并限制硅藻生长速率， 环境变化最终影响物种演替 溶解二氧化硅供应率。 辨别能力 在吸收的限制和生长的限制之间 速率对于我们理解营养限制至关重要， 水生系统 此外，还将进行实验， 研究硅藻生长速率的响应可能是一个关键 控制浮游植物对新营养盐的生产的因素 输入.

The determination of species specific growth rates in natural populations is one of the most sought after measurements in phytoplankton ecology. This research program will investigate the response of diatom growth rates to perturbations in dissolved silicate supplies using a fluorescent dye (Rhodamine 123). Diatoms incubated in Rhodamine 123 actively accumulate it into the silica deposition vesicle and Rhodamine 123 is co- deposited with silica. The resulting valves and girdle bands become fluorescent and provides a marker for cell division in diatoms; thus allowing for the estimation of species specific growth rates. Experiments will be performed to investigate the potential of low dissolved silicate concentrations to limit the uptake of dissolved silicate and to limit diatom growth rates, ultimately influencing species succession with changes in ambient dissolved silica supply rates. The ability to distinguish between the limitation of uptake and the limitation of growth rate is critical to our understanding of nutrient limitation in aquatic systems. In addition, experiments will be performed to investigate the response of diatom growth rate may be a key factor governing the phytoplankton production to new nutrient input.