PRF: Spatial Genetic Structure and Genetic Demography of Delphinium nelsonii

大多数植物种群的遗传结构将在 由于基因流动受到限制， 异质选择 然而，精细的尺度导致， 种群遗传结构的影响仍然存在 晦涩难懂。 拟议的研究旨在确定 群体精细尺度空间遗传结构的格局 山地草本植物翠雀花， 模式产生了。 将对两个群体进行采样 以在广泛范围内量化遗传结构， 空间尺度在每个种群中， 将使用同工酶对生命周期的各个阶段进行基因分型 电泳 成年人口结构将是 使用三种方法进行表征，并与 根据花粉和种子传播的经验措施估计 的距离. 遗传多样性和种群的偏差 不同生活史阶段之间的结构将用于 确定人口的时间和可能的机制 细分 将使用两种技术来估计影响 杂合性和近亲繁殖对后代生存的影响。 不同组合个体间的控制杂交 空间接近和遗传相似性。 三 适合度，种子设置，种子质量和种子重量的措施将是 在所产生的后代中测量。 方差分析将是 用于分解遗传相似性的关系， 电泳标记位点和空间邻近性对适合度的影响 outcrossing距离的影响。

Most plant populations will be structured genetically in space as a result of restricted gene flow and spatially heterogeneous selection. However, the fine scale causes and consequences of within population genetic structure remain obscure. The proposed research is aimed at determining the pattern of fine scale spatial genetic structure in populations of the montane herb Delphinium nelsonii and understanding how the pattern is produced. Two populations will be sampled hierarchically to quantify genetic structure at wide range of spatial scales. In each population, individuals from several stages in the life cycle will be genotyped using isozyme electrophoresis. Adult population structure will be characterized using three methods and compared to patterns estimated from empirical measures of pollen and seed dispersal distances. Deviations in genetic diversity and population structure among different life history stages will be used to identify the timing and possible mechanisms of population subdivision. Two techniques will be used to estimate the effects of heterozygosity and inbreeding on offspring survival. Controlled crosses between individuals of different combinations of spatial proximity and genetic similarity will be made. Three measures of fitness, seed set, seed mass, and seed weight will be measured in the resulting progeny. Analysis of variance will be used to decompose the relationship of genetic similarity at electrophoretic marker loci and spatial proximity on the fitness consequences of outcrossing distance.