Cellular and Molecular Events in Early Leech Development

早期水蛭发育中的细胞和分子事件

• 批准号: 9105713
• 负责人: David Weisblat
• 金额: $ 32.37万
• 依托单位: University of California-Berkeley
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-11-01 至 1994-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9105713&HistoricalAwards=false
• 关键词: Cellular; Molecular; Events; Early; Leech

Glossiphoniid leech embryos, such as those of the species Helobdella triserialis, are well suited for studying cellular aspects of early development, because they are relatively large and hardy, and because their blastomeres are both experimentally accessible and individually identifiable. Thus, experimental analyses can be carried out on developmentally equivalent cells from one embryo to the next. This proposal is to continue previous analyses of various aspects of leech development, including: the role of cytoplasmic determinants in determining early cleavage; the specification of mesodermal and ectodermal cell lines; fate determining interactions between initially equipotent cells; the continuing refinement of developmental cell lineages; the influence of a transient embryonic epithelium on morphogenesis. The experiments proposed will carry out these analyses at the cellular and molecular level, by incorporating the techniques of the "reverse genetic approach" in combination with those used previously, such as microinjection of cell lineage tracers and other materials into identified embryonic cells, single cell ablation and optical microscopy.

舌管目水蛭胚胎，例如 Helobdella triserialis非常适合研究细胞 早期发展的方面，因为它们相对较大， 和哈代，因为他们的卵裂球都是实验性的， 可访问和个人可识别。因此，实验 分析可以在发育等价细胞上进行 从一个胚胎到另一个胚胎。这项建议将继续下去， 先前对水蛭发育各个方面的分析， 包括：细胞质决定因素在决定 早期卵裂;中胚层和外胚层的特化 细胞系;决定命运的相互作用， 等能细胞;不断完善的发育细胞 谱系;短暂的胚胎上皮对 形态发生所提出的实验将执行这些 在细胞和分子水平上进行分析， “反向遗传方法”的技术结合 与以前使用的那些，如显微注射细胞 谱系示踪剂和其他材料， 细胞，单细胞消融和光学显微镜。