Molecular tools for fast switching of cellular events (Z01)

用于快速切换细胞事件的分子工具 (Z01)

• 批准号: 319511042
• 金额: --
• 依托单位: Department of Chemical Physiology and Biochemistry
• 依托单位国家: 德国
• 项目类别: CRC/Transregios
• 财政年份: 2016
• 资助国家: 德国
• 起止时间: 2015-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/319511042?language=en
• 关键词: Molecular; tools; fast; switching; cellular

Investigations of single switches but also complex signaling events are often experimentally accessible by inducing an artificial switch on the molecular level inside intact cells. Based on the extensive experience of the Schultz in developing tools for switching molecular events and its detection, the Z01 project will provide already existing tools such as caged lipids and chemical dimerizer systems and will develop new tools in close collaboration for the TRR partners. The novel probes will be based on small molecules but also genetically encoded constructs and cover a large variety of applications from endosomal trafficking to fluorescent probes that sense double-stranded RNA. Z01 will give consortium partners access to unique experimentation possibilities.

通过在完整细胞内的分子水平上诱导人工开关，通常可以对单个开关以及复杂信号事件进行实验研究。基于Schultz在开发开关分子事件及其检测工具方面的丰富经验，Z01项目将提供现有的工具，如笼状脂质和化学二聚体系统，并将与TRR合作伙伴密切合作开发新工具。这种新型探针将基于小分子，但也基于遗传编码结构，并涵盖从内体运输到检测双链RNA的荧光探针的各种应用。Z01将为合作伙伴提供独特的实验机会。