Genetic Analysis of Blue Light Perception and Signal Transduction Mechanisms in Plants

植物蓝光感知和信号转导机制的遗传分析

• 批准号: 9106697
• 负责人: Roger Hangarter
• 金额: --
• 依托单位: Ohio State University Research Foundation -DO NOT USE
• 依托单位国家: 美国
• 项目类别: Continuing grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-07-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9106697&HistoricalAwards=false
• 关键词: Genetic; Analysis; Blue; Light; Perception

The long-term objectives of this proposal are to determine at the cellular and molecular level how blue light is perceived by plants and transduced into growth responses. This project will investigate the physiological and genetic basis for blue light responses in higher plants using Arabidopsis as a model system. The response we will concentrate on is the inhibition of hypocotyl elongation caused by continuous blue light. Specific goals are to isolate a number of mutant strains that show altered responses to blue light and to use these mutants to characterize portions of the signal transduction pathway, perhaps the blue light receptor itself. Physiological analysis of the mutant plants will be conducted in order to identify what phases of the response are affected by the mutations. Interactions between various blue and red light responses share signal transduction events. Plants containing two or more light-response mutations will be genetically constructed and used in the investigation of the light-dependent signal transduction networks. The blue light-response mutants will be genetically characterized using complementation analysis to determine the minimum number of steps involved in signal transduction. Finally the mutations will be mapped to their chromosome locations followed by gene isolation, using T-DNA insertion mutants or by chromosome walking, in order to identify the proteins required for blue light signal transduction. Light not only supplies plants with the energy for photosynthesis, but also regulates nearly all stages of plant development, independent of photosynthesis. For a seedling to properly establish itself requires the coordinated action of numerous developmental pathways. Light, through the action of several photoreceptor systems, triggers many of the developmental changes essential for seedlings to make the transition from growing in the darkness of the soil to the light of the sun. The red/far red light receptor, phytochrome, has been the most intensively studied, whereas the nature of the blue and ultraviolet receptors is virtually unknown. This proposal seeks to identify and investigate the mechanisms by which plants perceive and respond to blue light and, thus, how plants adapt to different light conditions. The investigation of blue light response systems is likely to lead to important new insights into the physiological and molecular mechanisms controlling photomorphogenesis during seedling establishment.***//

该提案的长期目标是在细胞和分子水平上确定植物如何感知蓝光并转化为生长反应。 该项目将使用拟南芥作为模型系统，研究高等植物蓝光反应的生理和遗传基础。 我们将关注的反应是持续蓝光引起的下胚轴伸长的抑制。 具体目标是分离一些对蓝光反应发生改变的突变株，并利用这些突变株来表征信号转导途径的部分，也许是蓝光受体本身。 将对突变植物进行生理分析，以确定哪些反应阶段受到突变的影响。 各种蓝光和红光反应之间的相互作用共享信号转导事件。 含有两个或多个光响应突变的植物将被基因构建并用于光依赖性信号转导网络的研究。 将使用互补分析对蓝光响应突变体进行遗传表征，以确定信号转导中涉及的最少步骤数。 最后，突变将被映射到其染色体位置，然后使用 T-DNA 插入突变体或通过染色体行走进行基因分离，以便识别蓝光信号转导所需的蛋白质。 光不仅为植物提供光合作用的能量，而且还独立于光合作用调节植物发育的几乎所有阶段。 幼苗要正确地自我生长，需要众多发育途径的协调行动。 光通过多个感光系统的作用，引发许多对于幼苗从在黑暗的土壤中生长到在阳光下生长的转变至关重要的发育变化。 红/远红光受体（光敏色素）得到了最深入的研究，而蓝光和紫外线受体的性质实际上是未知的。 该提案旨在确定和研究植物感知和响应蓝光的机制，以及植物如何适应不同的光照条件。 蓝光响应系统的研究可能会对幼苗建立过程中控制光形态建成的生理和分子机制产生重要的新见解。***//