Cross talk and Metabolism in Escherichia coli

本项目旨在研究 调节相互作用，称为磷酸盐之间的串扰 (PHO)creABCD中的调节子和基因，以前称为phoM， 操纵子，并定义creABCD操纵子在 大肠埃希菌 该项目完全侧重于监管 涉及creABCD操纵子的相互作用。 PHO调节子编码 获得环境磷（P）的基因， 当外部（ext）无机磷酸盐（Pi）水平为 生长限制。creABCD操纵子，其表达不受影响 由Pi（ext），似乎响应于内部（int）的级别 有机磷形成的中央代谢，这可能是形成 底物水平磷酸化（糖酵解或TCA循环） 在其中Pi（int）被并入有机磷酸酯的步骤中。 调控相互作用的基因之间的预期，在一个 代谢途径; Pi（ext）到Pi（int）到有机磷（int） 其中Pi（ext）调节PHO调节子和有机磷酸盐（int） 可以调节PHO调节子和creABCD操纵子。等 串扰可以是底层控制机制的示例 对细胞代谢的一般（全局）控制至关重要， 生长;这在基因控制中尤其明显 在细菌中表达，但很可能发挥同样的作用 真核细胞中也是如此。

This project is to study the genetic and molecular basis of regulatory interactions termed cross talk between the phosphate (PHO) regulon and genes in the creABCD, formerly called phoM, operon and to define the biological role of the creABCD operon in Escherichia coli. The project focuses entirely on regulatory interactions involving the creABCD operon. The PHO regulon encodes genes for the acquisition of environmental phosphorus (P) and is induced when the external (ext) inorganic phosphate (Pi) level is growth limiting. The creABCD operon, whose expression is unaffected by Pi(ext), seems to respond to the level of an internal (int) organophosphate formed in central metabolism, which may be formed in substrate-level phosphorylation (in glycolysis or the TCA cycle) at a step where Pi(int) is incorporated into organophosphate. Regulatory interactions are expected between genes in a metabolic pathway; Pi(ext) to Pi(int) to organophosphate (int) in which Pi(ext) regulates the PHO regulon and organophosphate(int) may regulate both the PHO regulon and the creABCD operon. Such cross talk may be an example of an underlying control mechanisms essential for general (global) control of cell metabolism and growth; this is especially apparent in the control of gene expression in bacteria, but may quite possibly play the same role in eucaryotic cells too.