Bio-Imaging Analyzer (Phosphorimager)

这项提案涉及购买富士生物成像分析仪(磷酸盐成像仪)的申请，这是一种用于检测和定量各种生化实验中使用的放射性的新设备。这台仪器的定量能力将允许进行目前不可能进行的生化实验。与传统的使用X射线胶片的放射自显影技术相比，该技术的主要优点是增加了线性响应、动态范围和灵敏度，并缩短了曝光时间。该仪器提供了使用这种放射性同位素(如32P、35S、14C和125I)的实验的定量数据。怀特黑德研究所目前还没有这种类型的仪器。放射性样品的定量将由用户组在第8节详细说明的各种生化和分子生物学实验中进行。这项技术涉及使用存储荧光屏，该屏幕存储样品β粒子发射的能量。这种发射由仪器检测并存储在计算机硬盘上。然后，可以使用仪器提供的软件包访问和分析数据。一些目前不可能进行的定量生化实验将是可行的，因为磷光成像仪远远优于X射线胶片放射自显影。

This proposal involves a request to purchase the Fuji Bio-Imaging Analyzer (Phosphorimager), a new device for the detection and quantitation of radioactivity used in a variety of biochemical experiments. The quantitative capabilities of this instrument will allow biochemical experiments not currently possible. The primary advantages of this technology over conventional autoradiography using x-ray film are an increased linear response, dynamic range, and sensitivity, as well as reduced exposure time. The instrument provides quantitative data from experiments involving the use of this radioisotopes such as 32P, 35S, 14C, and 125I. An instrument of this type is not currently available at the Whitehead Institute. The quantitation of radioactive samples will be carried out by the user groups in a variety of biochemical and molecular biology experiment detailed in Section 8. The technology involves the use of storage phosphor screens which store energy form sample beta particle emission. This emission is detected by the instrument and stored on a computer hard disk. The data can then be accessed and analyzed using a software package provided with the instrument. A number of quantitative biochemical experiments not currently possible will be feasible because the Phosphorimager is vastly superior to X-ray film autoradiography.