Acquisition of a Mass Spectrometer

质谱计将主要用于目前使用外部设备进行质量分析的蛋白质测序和肽合成小组，以及用于开发DNA片段定位方法的小鼠基因组计划。激光解吸是一种非常优越的检测、分辨和测定分子质量的技术。与快速原子轰击、等离子体解吸、电喷雾或热喷雾等方法相比，激光解吸对样品进行电离有许多优点。Lasermat通常从1pmol的蛋白质和肽中提供1000 - 100,000个质量。质量观测质量的准确性与样品有关，但在质量为30,000时通常在0.1%以内。该仪器通过易于使用的个人电脑软件操作，控制数据收集和分析。该软件简化了非专业研究人员在仪器操作方面的培训，并消除了对质谱专家操作仪器和分析光谱的需要。

9217434 Matsudaira A mass spectrometer will be used primarily by groups involved in protein sequencing and peptide synthesis that currently use outside facilities for mass analysis and by the mouse genome project for developing methods for mapping DNA fragments. Laser desorption is a far superior technique for detecting, resolving, and determining the mass of a molecule. Ionization of the sample by laser desorption has many advantages over ionization by fast atom bombardment, plasma desorption, electrospray, or thermospray methods. The Lasermat routinely provides masses in the range 1,000 - 100,000 from 1 pmol of proteins and peptides. The accuracy of the mass observed mass is sample dependent but is generally within 0.1% at a mass of 30,000. The instrument is operated through easy-to-use software on a personal computer which controls data collection and analysis. The software simplifies the training of unspecialized researchers in the operation of the instrument and eliminates the need for a specialist in mass spectrometry to operate the instrument and analyze the spectra.